NFAT eGFP 报告慢病毒-NFAT eGFP Repo

报告基因 怎么选 EGFP 还是荧光素酶？成像和定量根本不是一回事

。两者也能组合——例如用 EGFP 做分选、用 Luciferase 做定量，各司其职。 项目落地前，再确认这一步 如果既想看定位又想定量，别在"二选一"上纠结，直接双标：荧光蛋白负责成像、荧光素酶负责读数。顺带一句，多报告基因载体在组合实验里省事很多——比如同一个慢病毒骨架上带 EGFP 标记加 Luciferase 定量，转进去一次就两头兼顾。还有些项目需要红绿多通道，那就提前规划好 mCherry / ZsGreen1 的搭配，避免后面串色返工。 再回答几个容易混淆的问题 要成像看定位选荧

Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice

-fms (Csf1r ) protooncogene. The c-fms gene is expressed in macrophage, trophoblast cell lineages, and to some extent granulocytes. A reporter gene construct containing 3.5-kb of 5′ flanking sequence and the downstream intron 2 of the c-fms gene

Introduction of shRNAs into Primary NK Cells with Lentivirus

differentiation, acquisition of self-tolerance, and induction of anti-tumor responses. We used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient gene transfer system to modify gene expression in primary murine NK cells