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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human AK1 Protein, N-His
- 库存:
999
- 供应商:
abinScience
- 规格:
100ug
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >85% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Accession | P0DTD1 |
| Protein length | Met1-Gly180 |
| Nature | Recombi\t |
| Predicted molecular weight | 21.94 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Alternative Names | Host translation inhibitor nsp1, Leader protein, Non-structural protein 1, nsp1, Replicase polyprotein 1ab, pp1ab, ORF1ab polyprotein |
| Species | 2 (SARS-CoV-2) |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
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文献和实验Cell Death Differ:中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制
病理发生。然而,SARS-CoV-2 感染引起肺细胞凋亡的机制仍不清楚。 2022 年 1 月 13 日,中山大学附属第五医院黄曦课题组在 Cell death & differentiation 上发表了题为 SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK 研究性论文。该研究证实了病毒膜(M)蛋白会通过靶向 BOK 分子,介导肺
信息 1、Recombinant Gene Expression : Reviews and Protocols Editor(s): Paulina Balbás1, Argelia Lorence2 Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico (2)Virginia Polytechnic Institute,State University,BlacksburgVA
如下:几丁质结合蛋白域―intein1―MCS―Intein2―几丁质结合蛋白。这个巧妙的设计有3重便利:N-端,C-端或者N-端和C-端同时融合短的内含肽序列。想做N端融合,你可以将目的基因替换MCS―Intein2―几丁质结合蛋白,如果想做C端融合表达,就可以将目的基因替换MCS前面的几丁质结合蛋白域―intein1―MCS,不用分2个载体;而且做过克隆的人会很有体会:很多时候双酶切载体,由于2个酶切位点都多克隆位点上,没有小片断切下来,所以是否成功双酶切就不好判断,如果用Twin载体就会切下一个片断
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