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- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
一年
- 英文名:
2-tert-Butyl-6-methylaniline
- 库存:
100
- 供应商:
科淼生物
- CAS号:
13117-94-7
- 规格:
1ml
产品中文名称:2-叔丁基-6-甲基苯胺
产品英文名称:2-tert-Butyl-6-methylaniline
CAS号:13117-94-7
纯度级别:≥98%(GC)(T)
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
Single Primer (Semi-Random) PCR
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI nuclease
Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
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