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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in 20 mM Tris-HCl pH 8.0, 100 mM NaCl,1mM EDTA, 4%trehalose,3654% mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Human RNF20 Protein, N-His-SUMO & C-Strep
- 库存:
999
- 供应商:
abinscience
- 规格:
50ug

| Product name | Recombinant Human RNF20 Protein, N-His-SUMO & C-Strep |
|---|---|
| Catalog No. | HP987012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | Q5VTR2 |
| Protein length | Asp906-Gly975 |
| Nature | Recombinant |
| Predicted molecular weight | 21.91 kDa |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | RING-type E3 ubiquitin transferase BRE1A, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A, RNF20, hBRE1, RING finger protein 20 |
| Species | Homo sapiens (Human) |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
Figure 19.20.1 The Strep/FLAG tandem affinity purification. (A ) N‐ and C‐terminal SF‐TAP tags (POI, protein of interest). (B ) Overview of both purification steps. (1) Purification by the tandem Strep‐tag II moiety: binding to Strep‐Tactin matrix
High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (x003E;10,000 constructs) and has resulted in the deposition of 112 unique structures
Purification of Human Multiprotein Complexes using OneSTrEP Technology
sufficient for visualising single protein bands by Coomassie Blue staining (Figure 1). The Strep -tag®II (SAWRHPQFGG) and its “double” sister, the One-STrEP-tag (tandem arrangement of Strep -tag®II, here called OneStrep), are reasonably small protein
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