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200314
XL10-Gold Ultracompetent Cells, 5 x 100 ul. For extremely demanding cloning, large plasmids and plasmid libraries. Efficiency: ≥5 x 109 transformants/µg pUC18 DNA Contains: XL10-Gold ultracompetent cells, pUC18 control plasmid, XL10-Gold b-mercaptoethanol mix
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| 感受态细胞应用 |
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| 生成未甲基化的 DNA |
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文献和实验Transformation of HB-101 Competent Cells
实验步骤 1. Thaw tubes of competent cells on ice, occasionally inverting gently to mix. 2. Pre-cool Falcon 2059 tubes on ice. 3. Aliquot 30 ul cells into each tube. 4. Add up to 10 ul DNA in TE or ligation
Transformation of Competent Cells
Transformation of Competent Cells A. Preparing competent cells. Inoculate 30 ml of SOB broth in a 250-ml flask with bacteria to be transformed from a single colony on a fresh plate.
FROZEN COMPETENT E. COLI CELLS
FROZEN COMPETENT E. COLI CELLS (Inoue et al., 1990 Gene 96:23) Inoculate a 5ml overnight of E.coli in LB+20 mM MgSO4. Next morning
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