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- AntibodySystem
- RVV28701
- 2025年07月12日
- ELISA
- Alpaca
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-SEBOV RNA-directed RNA polymerase L/Protein L Nanobody (SAA1489)
- 抗体英文名：
Anti-SEBOV RNA-directed RNA polymerase L/Protein L Nanobody (SAA1489)
- 浓度：
1 mg/ml
- 应用范围：
ELISA
- 宿主：
Alpaca
- 保质期：
1 year
- 级别：
科研级
- 库存：
999
- 供应商：
AntibodySystem
- 克隆性：
Monoclonal
- 亚型：
VHH-8His-Cys-tag
- 规格：
100ug
| 宿主 | Alpaca |
| 种属反应性 | Sudan ebolavirus (strain Human/Uganda/Gulu/2000) (SEBOV) (Sudan Ebolavirus) |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 浓度 | 1 mg/ml |
| 纯度 | >95% as determined by SDS-PAGE. |
| 克隆类型 | Monoclonal |
| 同种型 | VHH-8His-Cys-tag |
| 应用 | ELISA |
| 靶标 | L, RNA-directed RNA polymerase L, Protein L, Large structural protein, Replicase, Transcriptase |
| 纯化方式 | Purified by Nickel column. |
| 内毒素水平 | Please contact with the lab for this information. |
| 表达系统 | Mammalian Cells |
| Accession号 | Q5XX01 |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 克隆号 | SAA1489 |
| 备注 | For research use only. |
AntibodySystem products span research areas including 48 viruses, superbugs, parasites, cancer, Alzheimer's, Parkinson's, allergic and hypersensitivity reactions, immune suppression, and immune activation.
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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
μL) into new 1.5 ml centrifuge tube for RNA/DNA isolation. Remove any liquid from the original tube contains protein precipitate by invert the plate on a absorbent paper. Keep the tube contains protein pellet for protein isolation start from step 26
Reverse Transcription of RNA- 20l rxn
0.01l is the 1st strand derived from 0.00075 ug of Poly A+ RNA which is the equivalent of 0.15ug of total RNA (X-H''s method works out to use the eqivalent of 0.12ug of total RNA in the RT-PCR) Controls should include: 1) primers alone
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