- ￥1548
- AntibodySystem
- FHC27733
- France
- 2025年07月14日
- FCM
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Anti-Human CD3E Antibody (OKT3), APC
- 抗体英文名：
Anti-Human CD3E Antibody (OKT3), APC
- 浓度：
0.5 mg/ml
- 应用范围：
FCM
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
1 year
- 级别：
科研级
- 库存：
999
- 供应商：
AntibodySystem
- 克隆性：
Monoclonal
- 保存条件：
Store at 4°C for 12 months. Protect from light. Do not freeze.
- 亚型：
IgG2a, kappa
- 规格：
100T
| 宿主 | Mouse |
| 偶联物 | APC |
| 种属反应性 | Human |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| 浓度 | 0.5 mg/ml |
| 克隆类型 | Monoclonal |
| 同种型 | IgG2a, kappa |
| 应用 | FCM |
| 靶标 | T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain |
| 内毒素水平 | Please contact with the lab for this information. |
| 稳定性和存储 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 克隆号 | OKT3 |
| 备注 | For research use only. |
Currently, AntibodySystem's product lines cover multiple research areas, including 48 types of viruses and superbugs, parasites, tumors, Alzheimer's disease, Parkinson's disease, hypersensitivity and allergic reactions, immune suppression, and immune activation.
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文献和实验Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
a 5-10 µg/ml solution of anti-CD3e (145-2C11) in sterile PBS. Calculate the number of wells required for each experimental condition and consider triplicate samples for each condition. For example, to coat one-half plate (48 wells) 2.6 ml of antibody
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