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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-Mouse TCR αβ/alpha-beta TCR Antibody (H57-597), FITC
- 抗体英文名:
Anti-Mouse TCR αβ/alpha-beta TCR Antibody (H57-597), FITC
- 浓度:
0.5 mg/ml
- 应用范围:
FCM
- 宿主:
Rat
- 适应物种:
Mouse
- 保质期:
1 year
- 级别:
科研级
- 库存:
999
- 供应商:
AntibodySystem
- 克隆性:
Monoclonal
- 保存条件:
Store at 4°C for 12 months. Protect from light. Do not freeze.
- 亚型:
IgG2a, kappa
- 规格:
100T

| 宿主 | Rat |
| 偶联物 | FITC |
| 种属反应性 | Mouse |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. |
| 浓度 | 0.5 mg/ml |
| 克隆类型 | Monoclonal |
| 同种型 | IgG2a, kappa |
| 应用 | FCM |
| 靶标 | T-cell receptor beta-1 chain C region, T-cell receptor alpha chain C region |
| 内毒素水平 | Please contact with the lab for this information. |
| 稳定性和存储 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 克隆号 | H57-597 |
| 备注 | For research use only. |
Currently, AntibodySystem's product lines cover multiple research areas, including 48 types of viruses and superbugs, parasites, tumors, Alzheimer's disease, Parkinson's disease, hypersensitivity and allergic reactions, immune suppression, and immune activation.
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文献和实验板,利用 ELISA 方法测定抗体亲和力,可达到高通量检测效果。Anna Sergeeva 等(2011)在研究人类急性髓细胞样白血病的 TCR 样抗体工作中,用 HLA-A2-PR1 多肽复合物免疫小鼠,分离小鼠脾脏细胞。培养单克隆细胞,制备抗体。将 MHC-PR1 多肽复合物包被于酶联板上,进行同量化 ELISA 筛选。在 2850 个克隆中,筛选到一个(8F4)具备亲和力的抗体。推荐阅读:1. TCR Mimic Monoclonal Antibody Targets a Specific
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
by their expression of the coreceptor proteins CD4 and CD8 and their surface density of aβ T cell receptors (αβ TCR), ( 1 ). Three major subpopulations of T cells exist within the thymus that exemplify the progression of thymocytes along the CD4/CD8 developmental
小牛血清+0.1% 叠氮钠;建议使用时新鲜配制),制备成细胞悬浮液。2.取 25ul 细胞悬浮液滴加入微孔培养板或 FACS 试管。Staining Cocktail 制备3.制备 2X Staining Cocktail:FACS Buffer+ MHC 四聚体(建议稀释比例为 1:50~1:100)+ Anti-CD8/FITC 抗体或其他用作内参的抗体(请参考其说明书进行稀释)。染色孵育4.取 25ul 2X Staining Cocktail,滴加入步骤 2 中的微孔培养板或 FACS
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