3D421细胞

3D4/21 细胞；3D4/21 猪肺泡巨噬细胞；3D4/21 

Cell line name IPAM 3D4/21

Synonyms 3D4/21; IPAM-WT

Accession CVCL_0F14

Resource Identification Initiative To cite this cell line use: IPAM 3D4/21 (RRID:CVCL_0F14)

Comments Characteristics: SLA gene typing is SLA-1:04:01:01; SLA-2:04:02:01; SLA-DQA:02:03; SLA-DQB1:02:01 and SLA-DRB1:05:01 (PubMed=30241566).

Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.

Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).

Derived from site: In situ; Lung, alveolus; UBERON=UBERON_0002299.

Cell type: Alveolar macrophage; CL=CL_0000583.

Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)

Breed/subspecies: Landrace; VBO=VBO_0001161.

Hierarchy Parent: CVCL_0F12 (IPAM 3D4)

Children:

CVCL_ZE11 (IPAM 3D4/21/CD163) CVCL_ZE12 (mPAM-CD163-GFP) CVCL_VG78 (PAM-pCD163)

CVCL_D7HV (Ubigene 3D4/21 PLSCR3 KO)

Sex of cell Sex unspecified

Age at sampling 12W

Category Transformed cell line

Publications

PubMed=12088830; DOI=10.1016/S0166-0934(02)00085-X; PMCID=PMC7119708

Weingartl H.M., Sabara M.I., Pasick J., van Moorlehem E., Babiuk L.A.

Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility.

J. Virol. Methods 104:203-216(2002)

 

PubMed=28871180; DOI=10.1038/s41598-017-09948-x; PMCID=PMC5583235

Sanchez E.G., Riera E., Nogal M., Gallardo C., Fernandez P., Bello-Morales R., Lopez-Guerrero J.A., Chitko-McKown C.G., Richt J.A., Revilla Y.

Phenotyping and susceptibility of established porcine cells lines to African swine fever virus infection and viral production.

Sci. Rep. 7:10369-10369(2017)

 

PubMed=30241566; DOI=10.1186/s13567-018-0590-2; PMCID=PMC6151021

Le Q.V.-C., Le T.M., Cho H.-S., Kim W.-I., Hong K., Song H., Kim J.-H., Park C.

Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes.