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T25
3D4/21 细胞;3D4/21 猪肺泡巨噬细胞;3D4/21
Cell line name IPAM 3D4/21
Synonyms 3D4/21; IPAM-WT
Accession CVCL_0F14
Resource Identification Initiative To cite this cell line use: IPAM 3D4/21 (RRID:CVCL_0F14)
Comments Characteristics: SLA gene typing is SLA-1:04:01:01; SLA-2:04:02:01; SLA-DQA:02:03; SLA-DQB1:02:01 and SLA-DRB1:05:01 (PubMed=30241566).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).
Derived from site: In situ; Lung, alveolus; UBERON=UBERON_0002299.
Cell type: Alveolar macrophage; CL=CL_0000583.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Landrace; VBO=VBO_0001161.
Hierarchy Parent: CVCL_0F12 (IPAM 3D4)
Children:
CVCL_ZE11 (IPAM 3D4/21/CD163) CVCL_ZE12 (mPAM-CD163-GFP) CVCL_VG78 (PAM-pCD163)
CVCL_D7HV (Ubigene 3D4/21 PLSCR3 KO)
Sex of cell Sex unspecified
Age at sampling 12W
Category Transformed cell line
Publications
PubMed=12088830; DOI=10.1016/S0166-0934(02)00085-X; PMCID=PMC7119708
Weingartl H.M., Sabara M.I., Pasick J., van Moorlehem E., Babiuk L.A.
Continuous porcine cell lines developed from alveolar macrophages: partial characterization and virus susceptibility.
J. Virol. Methods 104:203-216(2002)
PubMed=28871180; DOI=10.1038/s41598-017-09948-x; PMCID=PMC5583235
Sanchez E.G., Riera E., Nogal M., Gallardo C., Fernandez P., Bello-Morales R., Lopez-Guerrero J.A., Chitko-McKown C.G., Richt J.A., Revilla Y.
Phenotyping and susceptibility of established porcine cells lines to African swine fever virus infection and viral production.
Sci. Rep. 7:10369-10369(2017)
PubMed=30241566; DOI=10.1186/s13567-018-0590-2; PMCID=PMC6151021
Le Q.V.-C., Le T.M., Cho H.-S., Kim W.-I., Hong K., Song H., Kim J.-H., Park C.
Analysis of peptide-SLA binding by establishing immortalized porcine alveolar macrophage cells with different SLA class II haplotypes.
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文献和实验*发表【中文论文】请标注:由博辉生物科技(广州)有限公司提供; *发表【英文论文】请标注:From Bohui Biological Technology (Guangzhou) Co., Ltd.
qp。在 CHO-MAb 分批补料培养的情况下,使用 5 倍浓缩氨基酸进料(``Hi'' 渗透法)会导致培养物的渗透压逐渐从 320 mOsm / kg 升高到约 420 mOsm / kg(图 2)与保持低渗透压(降至 280 mOsm / kg)的 1x 补料添加培养物('Lo' 渗透状态)相比。图 1. 渗透压对批次 MAb-CHO 培养的影响。(A)C44 和(B)C56 细胞系在不同分批培养渗透压下的活细胞浓度(VCC)与时间的关系。(C)qp 和(D)C44 和 C56 的最终 MAb
位升高),不知道我的这个结果是否可以说明gsk活性降低了?还是一定需要第九位的结果才能解释这个问题?似乎也有些人说216特异性不强,没有第九位有说服力,不是很能理解这其中的原因,再次感谢你! ylhuang0502 As for the GSK phosphorylation, it is clear now that, GSK serine phosphorylation (S9 for beta and S21 for alpha
4-1是NS1/1的亚系,抗8-Ag(20μg/ml),在HAT培养液中死亡。 2.小鼠P3-X63-Ag8。是Kohler和Milstein首次获得产生McAb杂交瘤所用的骨髓瘤细胞系。来源:它来自BALB/c小鼠的P3(MOPC21)骨髓瘤细胞系,分泌IgG1(K),抗8-AG(20μg/ml),在HAT培养液中不生长。 3.小鼠P3-X63-Ag8.653(1979年Kearney)。是P3-X63-Ag8的亚克隆,不可逆地丧失表达免疫球蛋白rl重链和K轻链的能力,其生长特性、融合
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