MC3T3E1细胞

MC3T3-E1 Subclone 14、MC3T3-E1 Subclone 14、MC3T3-E1 Subclone 14细胞、MC3T3-E1 Subclone 14细胞、MC3T3-E1 Subclone 14小鼠颅顶前骨细胞亚克隆14

Cell line name MC3T3-E1 Subclone 14

Synonyms MC3T3-E1 SUBCLONE 14

Accession CVCL_5437

Resource Identification Initiative To cite this cell line use: MC3T3-E1 Subclone 14 (RRID:CVCL_5437)

Comments Characteristics: Exhibits high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. Forms a well mineralized extracellular matrix (ECM) after 10 days (ATCC=CRL-2594).

Derived from site: In situ; Bone, calvaria; UBERON=UBERON_0004339.

Cell type: Osteoblast; CL=CL_0000062.

Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)

Breed/subspecies: C57BL/6.

Hierarchy Parent: CVCL_0409 (MC3T3-E1)

Children:

CVCL_C8WY (MC3T3-E1 Subclone 14-Luc) CVCL_E0Y9 (Ubigene MC3T3-E1 Subclone 14 Clk4 KO) CVCL_E0YA (Ubigene MC3T3-E1 Subclone 14 Egfr KO)

CVCL_E0YB (Ubigene MC3T3-E1 Subclone 14 Notum KO) CVCL_E0YC (Ubigene MC3T3-E1 Subclone 14 Npnt KO) CVCL_E0YD (Ubigene MC3T3-E1 Subclone 14 Tsc1 KO)

Sex of cell Sex unspecified

Age at sampling <1D

Category Spontaneously immortalized cell line

STR profile Source(s): Ubigene=YC-C026

 

Markers:

Mouse STR 4-2 20.3

Mouse STR 5-5 17

Mouse STR 6-4 18

Mouse STR 6-7 17

Mouse STR 12-1 17

Mouse STR 15-3 22.3

Mouse STR 18-3 15

Mouse STR X-1 28

 

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Publications

PubMed=10352097; DOI=10.1359/jbmr.1999.14.6.893

Wang D., Christensen K., Chawla K., Xiao G.-Z., Krebsbach P.H., Franceschi R.T.

Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential.

J. Bone Miner. Res. 14:893-903(1999)