HT29细胞

HT-29/HT-29细胞系/HT-29细胞株/HT-29人结肠腺癌细胞

Cell line name HT-29

Synonyms HT 29; HT29

Accession CVCL_0320

Resource Identification Initiative To cite this cell line use: HT-29 (RRID:CVCL_0320)

Comments Part of: AstraZeneca Colorectal cell line (AZCL) panel.

Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: JFCR39 cancer cell line panel.

Part of: KuDOS 95 cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: NCI-60 cancer cell line panel.

From: Memorial Sloan Kettering Cancer Center; New York; USA.

Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK 809.

Population: Caucasian.

Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).

Doubling time: 1.2 days (PubMed=6445228); 36.93 hours (PubMed=25944804); 24 hours (PubMed=25984343); 24 hours (PubMed=23546019); 21.87 +- 0.12 hours (PubMed=32927768); ~24 hours (CLS=300215); ~40-60 hours (DSMZ=ACC-299); 19.5 hours (NCI-DTP=HT-29); ~23 hours (PBCF).

Karyotypic information: Hypertriploid karyotype.

Microsatellite instability: Stable (MSS) (PubMed=9000147; PubMed=24042735; PubMed=24755471; PubMed=25926053; PubMed=28683746; Sanger).

Omics: Array-based CGH.

Omics: CNV analysis.

Omics: CRISPR phenotypic screen.

Omics: Deep exome analysis.

Omics: Extracellular vesicles proteome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep quantitative phosphoproteome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: Fluorescence phenotype profiling.

Omics: lncRNA expression profiling.

Omics: Metabolome analysis.

Omics: miRNA expression profiling.

Omics: N-glycan profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: Indicated in PubMed=11921276 as originating from an adenocarcinoma of the rectosigmoid part of the intestine, but is only indicated in the oldest publication (DOI=10.1007/978-1-4757-1647-4_5) as a colon adenocarcinoma.

Misspelling: HCT29; GEO=GSM206503.

Derived from site: In situ; Colon; UBERON=UBERON_0001155.

PubMed=22282976; DOI=10.1093/carcin/1.1.21

Day R.S. 3rd, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Mattern M.R.

Human tumor cell strains defective in the repair of alkylation damage.

Carcinogenesis 1:21-32(1980)

 

PubMed=7017212; DOI=10.1093/jnci/66.6.1003

Pollack M.S., Heagney S.D., Livingston P.O., Fogh J.

HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines.

J. Natl. Cancer Inst. 66:1003-1012(1981)

 

PubMed=7459858

Rousset M., Zweibaum A., Fogh J.

Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.

Cancer Res. 41:1165-1170(1981)

 

PubMed=6220172

Dracopoli N.C., Fogh J.

Polymorphic enzyme analysis of cultured human tumor cell lines.

J. Natl. Cancer Inst. 70:469-476(1983)

 

PubMed=6825208; DOI=10.1093/carcin/4.2.199

Yarosh D.B., Foote R.S., Mitra S., Day R.S. 3rd

Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.

Carcinogenesis 4:199-205(1983)

 

PubMed=6500159; DOI=10.1159/000163283

Gershwin M.E., Lentz D., Owens R.B.

Relationship between karyotype of tissue culture lines and tumorigenicity in nude mice.

Exp. Cell Biol. 52:361-370(1984)

 

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720

Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.

Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.

Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

 

PubMed=3518877; DOI=10.3109/07357908609038260

Fogh J.

Human tumor lines for cancer research.

Cancer Invest. 4:157-184(1986)

 

PubMed=3472642; DOI=10.1016/0165-4608(87)90267-6

Chen T.-R., Drabkowski D.J., Hay R.J., Macy M.L., Peterson W.D. Jr.

WiDr is a derivative of another colon adenocarcinoma cell line, HT-29.

Cancer Genet. Cytogenet. 27:125-134(1987)

 

PubMed=3335022

Alley M.C., Scudiero D.A., Monks A., Hursey M.L., Czerwinski M.J., Fine D.L., Abbott B.J., Mayo J.G., Shoemaker R.H., Boyd M.R.

Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay.

Cancer Res. 48:589-601(1988)