HTR-8/SVneo 人绒毛膜滋养层细胞

HTR-8/SVneo/HTR-8/SVneo细胞系/HTR-8/SVneo细胞株/HTR-8/SVneo人胎盘滋养细胞

Cell line name HTR-8/SVneo

Synonyms HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn

Accession CVCL_7162

Resource Identification Initiative To cite this cell line use: HTR-8/SVneo (RRID:CVCL_7162)

Comments Part of: ENCODE project common cell types; tier 3.

Characteristics: Useful to study trophoblast and placental biology (ATCC=CRL-3271).

Characteristics: Contains both epithelial and mesenchymal-like cell populations (PubMed=28161053).

Karyotypic information: Near triploid karyotype (PubMed=33096371).

Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.

Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSV3-neo).

Omics: DNA methylation analysis.

Derived from site: In situ; Trophoblast; UBERON=UBERON_0000088.

Source(s): ATCC=CRL-3271; PubMed=33810901

 

Markers:

Amelogenin X

CSF1PO 12

D1S1656 16,17.3

D2S441 11

D2S1338 23,24

D3S1358 16

D5S818 12

D7S820 12

D8S1179 13,15

D10S1248 16

D12S391 19

D13S317 9,12

D16S539 13

D18S51 13

D19S433 14,15

D21S11 29,30

D22S1045 15

FGA 21,23

Penta D 9,12

Penta E 7,13

SE33 25.2,27.2

TH01 6,9.3

TPOX 8

vWA 13,18 (ATCC=CRL-3271)

18 (PubMed=33810901)

PubMed=7684692; DOI=10.1006/excr.1993.1139

Graham C.H., Hawley T.S., Hawley R.G., MacDougall J.R., Kerbel R.S., Khoo N., Lala P.K.

Establishment and characterization of first trimester human trophoblast cells with extended lifespan.

Exp. Cell Res. 206:204-211(1993)

 

PubMed=23325432; DOI=10.1101/gr.147942.112; PMCID=PMC3589544

Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.

Dynamic DNA methylation across diverse human cell lines and tissues.

Genome Res. 23:555-567(2013)

 

PubMed=28161053; DOI=10.1016/j.placenta.2016.12.007

Abou-Kheir W., Barrak J., Hadadeh O., Daoud G.

HTR-8/SVneo cell line contains a mixed population of cells.

Placenta 50:1-7(2017)

 

PubMed=33096371; DOI=10.1016/j.placenta.2020.10.011

Weber M., Weise A., Vasheghani F., Gohner C., Fitzgerald J.S., Liehr T., Markert U.R.

Cytogenomics of six human trophoblastic cell lines.

Placenta 103:72-75(2021)

 

PubMed=33810901; DOI=10.1016/j.placenta.2021.02.022

Pastuschek J., Nonn O., Gutierrez-Samudio R.N., Murrieta-Coxca J.M., Muller J., Sanft J., Huppertz B., Markert U.R., Groten T., Morales-Prieto D.M.

Molecular characteristics of established trophoblast-derived cell lines.

Placenta 108:122-133(2021)