相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 询价记录
- 文献和实验
- 技术资料

Cell Counting Kit-8 (CCK-8)
Apexbio 细胞增殖试剂盒(CCK-8)
K1018
1x1ml (100 tests)
1x5ml (500 tests)
6x5ml (3000 tests)
20x5ml (10000 tests)
Product Citation
1. Sandro Matosevic, et al. "synNotch-programmed iPSC-derived NK cells usurp TIGIT and CD73 activities for glioblastoma
therapy." Nat Commun. 2024 Mar 1;15(1):1909. PMID: 38429294
2. Rongrong Xuan, et al. "Gut microbiota-derived trimethylamine N-Oxide: a novel target for the treatment of preeclampsia.
" Gut Microbes. 2024 Jan-Dec;16(1):2311888. PMID: 38351748
3. Chun-Wei Chen, et al. "A novel class of inhibitors that disrupts the stability of integrin heterodimers identified by CRISPR-tiling
-instructed genetic screens." Nat Struct Mol Biol. 2024 Mar;31(3):465-475. PMID: 38316881
4. Fenghuang Zhan, et al. "Bispecific BCMA/CD24 CAR-T cells control multiple myeloma growth." Nat Commun. 2024 Jan 19;15
(1):615. PMID: 38242888
5. Sanhong Liu, Weidong Zhang, et al. "Benzosceptrin C induces lysosomal degradation of PD-L1 and promotes antitumor
immunity by targeting DHHC3." ell Rep Med. 2024 Feb 20;5(2):101357. PMID: 38237597
6. Xiaodan Meng, et al. "LncRNA AL139294.1 can be transported by extracellular vesicles to promote the oncogenic behaviour
of recipient cells through activation of the Wnt and NF-κB2 pathways in non-small-cell lung cancer." J Exp Clin Cancer Res.
2024 Jan 16;43(1):20. PMID: 38229152
Description
Our product Cell Counting Kit-8 (CCK-8) provide a more convenient and sensitive method for the research of cell number
determination and cell proliferationtotoxicity assay than previous ways. The kit utilizes a highly water-soluble tetrazolium salt,
WST-8, which produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. The amount of
the formazan generated by dehydrogenases is directly in proportion to the numbers of living cells. The detection sensitivity by
CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Features
More Sensitive than MTT, MTS, or WST-1
No Toxicity to Cell
Simpler Steps, No organic solvents required
Stable One Bottle Solution, ready-to-use
Storage
Store at -20°C away from light.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
询价记录
暂无询价记录
文献和实验一、实验原理 细胞活力检测试剂盒(CCK-8) 可用于简便而准确的细胞增殖和毒性分析。 其基本原理为:该试剂中含有WST-8【化学名:2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯)-2H-四唑单钠盐】,在电子载体1-甲氧基-5-甲基吩嗪鎓硫酸二甲酯(1-Methoxy PMS)的作用下被细胞中的脱氢酶还原为具有高度水溶性的黄色甲瓒产物(Formazan dye)。生成的甲瓒物的数量与活细胞的数量成正比。因此可利用这一特性直接进行细胞增殖和毒性分析。CCK-8法
细胞增殖实验看起来方法很多,但不同检测实际上观察的是不同层面的变化。CCK-8和ATP生物发光主要反映活细胞的代谢状态,EdU直接标记DNA合成,克隆形成观察长期持续增殖能力,Ki-67可定位组织或细胞中的增殖群体,流式细胞周期则显示细胞在G0/G1、S和G2/M期的分布。 本文围绕这6种常用方法,整理各自的检测原理、典型操作、适用场景及优缺点,重点区分不同结果究竟代表什么。 一、六种方法分别测到了什么? 二、CCK-8:最常用的细胞
在细胞培养实验过程中,大家是不是总会好奇别人家的实验总是做的又快又好,而自己做的实验又慢又难得做出自己满意的结果,搞得一天到晚也挺忙的,心想:我也很努力很认真啊,为啥效率总提不起来呢?其实,有时候并不是自己不努力,只是没有找对方法。做实验可不能光是埋头苦干,还要学会运用合适的检测试剂盒,这样就能事半功倍。如果你还在用 MTT,那就真的 OUT 了,MTT 花 4 小时才能得到的结果用 CCK-8 只需 1 小时,CCK-8 可用于细胞增殖和毒性分析,同 MTT 法相比,CCK-8 即开即用
技术资料暂无技术资料 索取技术资料







