HCT-8细胞

HCT-8/HCT-8细胞系/HCT-8细胞株/HCT-8人结肠癌细胞

Cell line name HCT 8

Synonyms HCT-8; HCT8

Accession CVCL_2478

Resource Identification Initiative To cite this cell line use: HCT 8 (RRID:CVCL_2478)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: MD Anderson Cell Lines Project.

Population: Caucasian.

Virology: Not susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).

Doubling time: 27.49 hours (PubMed=25944804).

Microsatellite instability: Instable (MSI) (PubMed=10674020; PubMed=24755471; PubMed=25926053).

Omics: N-glycan profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Misspelling: HCT 18; Note=Occasionally.

Derived from site: In situ; Colon; UBERON=UBERON_0001155.

Cell type: Epithelial cell of colon; CL=CL_0011108.

Sequence variations

Mutation; HGNC; 583; APC; Simple; p.Arg727Met (c.2180G>T); Zygosity=Unspecified (PubMed=24755471).

Mutation; HGNC; 583; APC; Simple; p.Lys993Asn (c.2979G>T); Zygosity=Unspecified (PubMed=24755471).

Mutation; HGNC; 583; APC; Simple; p.Ile1417Leufs*2 (c.4248delC); Zygosity=Unspecified (PubMed=24755471).

Mutation; HGNC; 583; APC; Simple; p.Arg2166Ter (c.6496C>T); ClinVar=VCV000438883; Zygosity=Unspecified (PubMed=24755471).

Mutation; HGNC; 2514; CTNNB1; Simple; p.Val79Ile (c.235G>A); Zygosity=Unspecified (PubMed=24755471).

Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).

Mutation; HGNC; 8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).

Mutation; HGNC; 8975; PIK3CA; Simple; p.Asp549Asn (c.1645G>A); ClinVar=VCV000375900; Zygosity=Unspecified (PubMed=20570890; PubMed=24755471).

Mutation; HGNC; 6768; SMAD2; Simple; p.Pro164fs*8 (c.490_491insA); Zygosity=Unspecified (PubMed=24755471).

Mutation; HGNC; 6769; SMAD3; Simple; p.Val244Ile (c.730G>A); ClinVar=VCV000919044; Zygosity=Unspecified (PubMed=24755471).

HLA typing Source: PubMed=77569

Class I

HLA-A A*03,09

HLA-B B*08

 

Source: PubMed=26589293

Class I

HLA-A A*02:01,24:02

HLA-B B*08:01,35:01

HLA-C C*04:01,07:01

Disease Colon adenocarcinoma (NCIt: C4349)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Parent: CVCL_3449 (__Parent_cell_line_of_DLD-1/HCT 8/HCT 15/HRT-18)

Children:

CVCL_5679 (HCT-8/E11) CVCL_5945 (HCT-8/R1) CVCL_5947 (HCT-8/S10)

CVCL_5682 (HCT-8/S11) CVCL_A9CF (HCT-8/VCR) CVCL_A9CQ (HCT-8-Luc2-tdT)

CVCL_A9CR (HCT-8-mCherry) CVCL_A9CS (HCT-8-tdT) CVCL_S851 (HCT-8R)

Sex of cell Male

Age at sampling 67Y

Category Cancer cell line

STR profile Source(s): AddexBio=C0009017/4907; CCRID; CLS=300210; KCLB=10244; PubMed=25877200; PubMed=25926053

 

Markers:

Amelogenin X (CCRID; PubMed=25877200)

X,Y (AddexBio=C0009017/4907; CLS=300210; KCLB=10244; PubMed=25926053)

CSF1PO 12

D2S1338 17,25

D3S1358 17

D5S818 13

D7S820 10,11.3 (CCRID; PubMed=25877200)

10,11.3,12 (AddexBio=C0009017/4907)

10,12 (CLS=300210; KCLB=10244; PubMed=25926053)

D8S1179 15

D13S317 8,11

D16S539 12,13

D18S51 11,17

D19S433 14,16

D21S11 29,32.2

FGA 22

Penta D 9,14

Penta E 7,14

TH01 7,9.3

TPOX 8,11

vWA 18,19

 

Run an STR similarity search on this cell line

Publications

PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x

Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.

Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.

Tissue Antigens 11:279-286(1978)

 

PubMed=8422623; DOI=10.1002/1097-0142(19930115)71:2<315::AID-CNCR2820710208>3.0.CO;2-B

Tibbetts L.M., Doremus C.M., Tzanakakis G.N., Vezeridis M.P.

Liver metastases with 10 human colon carcinoma cell lines in nude mice and association with carcinoembryonic antigen production.

Cancer 71:315-321(1993)

 

PubMed=9809040; DOI=10.1016/S0165-4608(98)00081-8

Vermeulen S.J., Chen T.-R., Speleman F., Nollet F., Van Roy F.M., Mareel M.M.

Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?

Cancer Genet. Cytogenet. 107:76-79(1998)

 

PubMed=10674020; DOI=10.1016/S0959-8049(99)00206-3

Ku J.-L., Yoon K.-A., Kim D.-Y., Park J.-G.

Mutations in hMSH6 alone are not sufficient to cause the microsatellite instability in colorectal cancer cell lines.

Eur. J. Cancer 35:1724-1729(1999)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192; PMCID=PMC2943514

Janakiraman M., Vakiani E., Zeng Z.-S., Pratilas C.A., Taylor B.S., Chitale D., Halilovic E., Wilson M., Huberman K., Ricarte Filho J.C.M., Persaud Y., Levine D.A., Fagin J.A., Jhanwar S.C., Mariadason J.M., Lash A., Ladanyi M., Saltz L.B., Heguy A., Paty P.B., Solit D.B.

Genomic and biological characterization of exon 4 KRAS mutations in human cancer.

Cancer Res. 70:5901-5911(2010)

 

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013

Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.

Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.

Cancer Res. 74:3238-3247(2014)

 

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)

 

PubMed=25877200; DOI=10.1038/nature14397

Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.

A resource for cell line authentication, annotation and quality control.

Nature 520:307-311(2015)

 

PubMed=25926053; DOI=10.1038/ncomms8002

Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.

The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.

Nat. Commun. 6:7002.1-7002.10(2015)

 

PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457

Bazzocco S., Dopeso H., Carton-Garcia F., Macaya I., Andretta E., Chionh F., Rodrigues P., Garrido M., Alazzouzi H., Nieto R., Sanchez A., Schwartz S. Jr., Bilic J., Mariadason J.M., Arango D.

Highly expressed genes in rapidly proliferating tumor cells as new targets for colorectal cancer treatment.

Clin. Cancer Res. 21:3695-3704(2015)