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T25
C17.2 /C17.2 细胞系/C17.2 细胞株/C17.2 小鼠神经干细胞
Cell line name C17.2
Synonyms C17
Accession CVCL_4511
Resource Identification Initiative To cite this cell line use: C17.2 (RRID:CVCL_4511)
Comments Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P01110; Avian myelocytomatosis virus MC29 v-Myc.
Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: v-Myc.
Derived from site: In situ; Brain, cerebellum; UBERON=UBERON_0002037.
Cell type: Neural progenitor cell; CL=CL_0011020.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6 x CD-1.
Sex of cell Sex unspecified
Age at sampling 0D
Category Transformed cell line
Publications
PubMed=2139634; DOI=10.1002/glia.440030207
Weinstein D.E., Shelanski M.L., Liem R.K.H.
C17, a retrovirally immortalized neuronal cell line, inhibits the proliferation of astrocytes and astrocytoma cells by a contact-mediated mechanism.
Glia 3:130-139(1990)
PubMed=2307979; DOI=10.1002/neu.480210209
Ryder E.F., Snyder E.Y., Cepko C.L.
Establishment and characterization of multipotent neural cell lines using retrovirus vector-mediated oncogene transfer.
J. Neurobiol. 21:356-375(1990)
PubMed=22542584; DOI=10.1016/j.tiv.2012.04.020
Lundqvist J., El Andaloussi-Lilja J., Svensson C., Gustafsson Dorfh H., Forsby A.
Optimisation of culture conditions for differentiation of C17.2 neural stem cells to be used for in vitro toxicity tests.
Toxicol. In Vitro 27:1565-1569(2013)
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
掌握住最新的科研资讯,你就是科研界最机智的崽~① PNAS:让我们来听听细胞之间的对话康涅狄格大学的研究人员近日开发出一种新技术,能够实时记录细胞通讯,从而帮助人们更深入地了解细胞分泌的动态,以及细胞如何修复组织。这项成果于近日发表在《美国国家科学院院刊》(PNAS)杂志上。“这项成果解决了困扰生物学的一个基本问题 – 测定细胞之间如何相互沟通,”共同作者Yashir Suhail表示。“通过一种独特的方式来检测细胞之间如何交流,这个平台将开辟新的研究领域。”原文检索Dynamic
Silencing of Rho-GDI by RNAi Promotes the Differentiation of Neural Stem Cells
morphological changes in neural stem cell line C17.2. More important, RT-PCR confirmed that RNAi-mediated downregulation of Rho-GDIγ decreased expression of Rho-GDIγ-regulated genes RhoA and slightly increased expression of Rac1. Further, immunochemical staining
C17 H20 O6 N4 ,为维生素 B复合物中的耐热性生长促进因子,也就是具有维生素 B2 作用的物质,也曾称为乳黄素( lactoflavin)。和维生素 G为同一物质。它的溶液在 260、 375、 450毫微米处有吸收带,发黄绿色荧光。如果被亚硫酸氢盐等还原,咯嗪核的氮接受 2原子氢,变成无色核黄素,在空气中能自动氧化回复成黄素。氧化还原电位 E0 =-0.185v( pH=7)。如在碱性介质中用光照射,可被切掉侧链成为光黄素,在酸性或中性介质中可光解成光色
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