• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Silencing of Rho-GDI by RNAi Promotes the Differentiation of Neural Stem Cells

        互联网

        487
        RNA interference (RNAi) technology is one of the main means in the study of stem cell differentiation. This study describes Rho-GDIγ function during the differentiation of neural stem cells by using RNAi. Rho-GDIγ belongs to the Rho-GDI protein family, which is expressed at high level throughout the brain. Although it exists in neuronal population, its physiological function is poorly understood. By using RNAi technology to downregulate expression of Rho-GDIγ, we found distinct morphological changes in neural stem cell line C17.2. More important, RT-PCR confirmed that RNAi-mediated downregulation of Rho-GDIγ decreased expression of Rho-GDIγ-regulated genes RhoA and slightly increased expression of Rac1. Further, immunochemical staining indicated that downregulation of Rho-GDIγ increased the tendency of C17.2 cells to differentiate. These data strongly suggest that Rho-GDIγ plays a key role in the differentiation of neural stem cells.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序