CHP-212细胞

CHP212/CHP212细胞系/CHP212细胞株/CHP212脑神经细胞瘤细胞

Cell line name CHP-212

Synonyms CHP 212; CHP212; NB9; NB-9; Children's Hospital of Philadelphia-212

Accession CVCL_1125

Secondary accession CVCL_A797

Resource Identification Initiative To cite this cell line use: CHP-212 (RRID:CVCL_1125)

Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).

Part of: COSMIC cell lines project.

Part of: RAS genetic alteration cell panel (ATCC TCP-1031).

Population: Caucasian.

Characteristics: Intermediate type (I-type) (PubMed=15720811).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: Array-based CGH.

Omics: CRISPR phenotypic screen.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: DNA methylation analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Derived from site: In situ; Brain; UBERON=UBERON_0000955.

Sequence variations

Mutation; HGNC; 7989; NRAS; Simple; p.Gln61Lys (c.181C>A); ClinVar=VCV000073058; Zygosity=Heterozygous (PubMed=12068308; ATCC=CRL-2273; Cosmic-CLP=906820; DepMap=ACH-000120).

HLA typing Source: PubMed=26589293

Class I

HLA-A A*02:01,24:02

HLA-B B*07:07,58:05

HLA-C C*07:02,07:02

Genome ancestry Source: PubMed=30894373

 

Origin % genome

African 2.16

Native American 0

East Asian, North 1.09

East Asian, South 0.43

South Asian 0.86

European, North 70.26

European, South 25.19

Disease Neuroblastoma (NCIt: C3270)

Neuroblastoma (ORDO: Orphanet_635)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Sex of cell Male

Age at sampling 1Y8M

Category Cancer cell line

STR profile Source(s): ATCC=CRL-2273; Cosmic-CLP=906820; PubMed=25877200; RCB=RCB0477

 

Markers:

Amelogenin X,Y

CSF1PO 12

D2S1338 22,25

D3S1358 15,17

D5S818 10,12

D7S820 11

D8S1179 11,13

D13S317 8 (RCB=RCB0477)

8,13 (ATCC=CRL-2273; Cosmic-CLP=906820; PubMed=25877200)

D16S539 13

D18S51 14,16

D19S433 12,15

D21S11 27,29

FGA 20

Penta D 9,10

Penta E 5,15

TH01 6 (ATCC=CRL-2273; Cosmic-CLP=906820; PubMed=25877200)

6,7 (RCB=RCB0477)

TPOX 8,11

vWA 15,18

 

Run an STR similarity search on this cell line

Publications

PubMed=6113887

Schlesinger H.R., Rorke-Adams L.B., Jamieson R., Hummeler K.

Neuronal properties of neuroectodermal tumors in vitro.

Cancer Res. 41:2573-2575(1981)

 

PubMed=7139592; DOI=10.1016/0165-4608(82)90105-4

Gilbert F., Balaban-Malenbaum G.B., Moorhead P.S., Bianchi D., Schlesinger H.R.

Abnormalities of chromosome 1p in human neuroblastoma tumors and cell lines.

Cancer Genet. Cytogenet. 7:33-42(1982)

 

DOI=10.1016/B978-0-12-008304-6.50015-4

Biedler J.L., Meyers M.B., Spengler B.A.

Homogeneously staining regions and double minute chromosomes, prevalent cytogenetic abnormalities of human neuroblastoma cells.

(In book chapter) Advances in cellular neurobiology, Vol. 4; Fedoroff S., Hertz L. (eds.); pp.267-307; Academic Press; New York; USA (1983)

 

DOI=10.1016/B978-0-12-333530-2.50006-X

Israel M.A., Thiele C.J.

Tumor cell lines of the peripheral nervous system.

(In book chapter) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York; USA (1994)

 

DOI=10.1007/0-306-46872-7_2

Thiele C.J.

Neuroblastoma.

(In book chapter) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters J.R.W., Palsson B.O. (eds.); pp.21-53; Kluwer Academic Publishers; New York; USA (1999)

 

PubMed=12068308; DOI=10.1038/nature00766

Davies H.R., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.

Mutations of the BRAF gene in human cancer.

Nature 417:949-954(2002)

 

PubMed=15720811; DOI=10.1593/neo.04310; PMCID=PMC1531688

Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.

Characteristics of stem cells from human neuroblastoma cell lines and in tumors.

Neoplasia 6:838-845(2004)

 

PubMed=15892104; DOI=10.1002/gcc.20198

Mosse Y.P., Greshock J., Margolin A.A., Naylor T., Cole K.A., Khazi D., Hii G., Winter C., Shahzad S., Asziz M.U., Biegel J.A., Weber B.L., Maris J.M.

High-resolution detection and mapping of genomic DNA alterations in neuroblastoma.

Genes Chromosomes Cancer 43:390-403(2005)

 

PubMed=18724359; DOI=10.1038/nature07261; PMCID=PMC2672043

Mosse Y.P., Laudenslager M., Longo L., Cole K.A., Wood A., Attiyeh E.F., Laquaglia M.J., Sennett R., Lynch J.E., Perri P., Laureys G., Speleman F., Kim C., Hou C.-P., Hakonarson H., Torkamani A., Schork N.J., Brodeur G.M., Tonini G.P., Rappaport E., Devoto M., Maris J.M.

Identification of ALK as a major familial neuroblastoma predisposition gene.

Nature 455:930-935(2008)

 

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113

Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.

Signatures of mutation and selection in the cancer genome.

Nature 463:893-898(2010)

 

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662

Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.

A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.

Cancer Res. 70:2158-2164(2010)

 

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027

Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.

The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.

Nature 483:603-607(2012)

 

PubMed=25485619; DOI=10.1038/nbt.3080

Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.

A comprehensive transcriptional portrait of human cancer cell lines.

Nat. Biotechnol. 33:306-312(2015)