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T25
H4-II-E-C3/H4-II-E-C3细胞系/H4-II-E-C3细胞株/H4-II-E-C3大鼠肝癌细胞
Cell line name H4-II-E-C3
Synonyms H4IIEC3; H4-IIE-C3; H4-II-EC3; H-II-E-C3
Accession CVCL_0285
Resource Identification Initiative To cite this cell line use: H4-II-E-C3 (RRID:CVCL_0285)
Comments Omics: Transcriptome analysis by microarray.
Misspelling: H4-I-E-C3; Note=Occasionally.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Disease Rat hepatocellular carcinoma (NCIt: C60416)
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: AxC; RS=RS:0000369.
Hierarchy Parent: CVCL_0284 (H4-II-E)
Children:
CVCL_5125 (Fu5-5) CVCL_1B44 (H35R0.3) CVCL_4211 (H4S)
CVCL_3512 (H4TG) CVCL_4212 (H5) CVCL_0377 (KRC-7 [Rat hepatoma])
Sex of cell Male
Category Cancer cell line
Publications
PubMed=4404256
DeLuca C., Massaro E.J., Cohen M.M.
Biochemical and cytogenetic characterization of rat hepatoma cell lines in vitro.
Cancer Res. 32:2435-2440(1972)
PubMed=24386269; DOI=10.1371/journal.pone.0083751; PMCID=PMC3875483
Permenter M.G., Dennis W.E., Sutto T.E., Jackson D.A., Lewis J.A., Stallings J.D.
Exposure to cobalt causes transcriptomic and proteomic changes in two rat liver derived cell lines.
PLoS ONE 8:E83751-E83751(2013)
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
E.Z.N.A. Plant RNA Midi Protocol II (for difficult samples)
ml collection tube (Not supplied), and add 3.5 ml RNA Wash Buffer II diluted with ethanol. Centrifuge at 4,000 x g for 5 minutes at room temperature and discard flow-through. Reuse the collection tube in step 11. 11. Wash column with a second 3 ml of RNA
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
yields. 3. Transfer the sample to a 1.5 ml centrifuge tube. Add 500 ul Solution II and mix gently but throughly by inverting and rotating the tube 7-10 times to obtain a cleared lysate. This may require a 2-3 min incubation at room temperature
Methods for Assessing Autophagy and Autophagic Cell Death Cytosolic LC3 Ratio as a Sensitive Index of Macroautophagy in Isolated Rat Hepatocytes and H4-II-E Cells 本文经作者系 @丁香园中级站友 biofish。
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