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维百奥生物提供ZENOGEN PHARMA CO., LTD.公司，全系列细胞冻存液
STEM-CELLBANKER® GMP grade 细胞冻存液
STEM-CELLBANKER® DMSO Free GMP grade 细胞冻存液
STEM-CELLBANKER® EX GMP grade 细胞冻存液
HSC-BANKER® GMP grade 细胞冻存液
CELLBANKER® 1 细胞冻存液
CELLBANKER® 2 细胞冻存液
CELLOTION® 细胞冻存液
STEM-CELLBANKER® GMP grade 细胞冻存液
STEM-CELLBANKER® GMP grade
(Chemically defined cryopreservation solution)
Description:
STEM-CELLBANKER® is a chemically defined freezing solution with optimized formulation for stem cells and iPS cells storage
as well as other valuable cells. Furthermore, recent data supports it's ability of cryopreservation for tissues. It is completely free
of serum and animal derived component, and contains only USP, EP, JP graded ingredients or JP excipients. It is ready-to-use
with simple protocol while provides a safer and promising cryopreservation of stem cells and iPS cells.
STEM-CELLBANKER® GMP grade is manufactured in a facility compliant with JP, EU, US and PIC/S GMP guidelines and the
production and the quality are controlled in compliance with JP GMP guidelines.
Package:
20ml, 100ml / bottle
STEM-CELLBANKER® DMSO Free GMP grade 细胞冻存液
STEM-CELLBANKER® DMSO Free GMP grade
(Chemically defined cryopreservation solution)
Description:
STEM-CELLBANKER® DMSO Free GMP grade is a chemically defined freezing solution that does not contain
DMSO as an anti-freezing agent. It was developed for customers who do not prefer to use DMSO-containing
cryopreservation solution due to a variety of their intended uses.
Package:
20ml, 100ml / bottle
STEM-CELLBANKER® EX GMP grade 细胞冻存液
STEM-CELLBANKER® EX GMP grade
(Chemically defined cryopreservation solution)
Description:
STEM-CELLBANKER® EX GMP grade (SCB EX) is a cell cryopreservation solution that expands the possibilities of cell therapy.
HSC-BANKER® GMP grade 细胞冻存液
HSC-BANKER® GMP grade
(For cryopreserving hematopoietic stem cell)
Description
HSC-BANKER® GMP grade is an optimized cryopreservation medium for hematopoietic stem cells.
CELLBANKER® 1 细胞冻存液
CELLBANKER® 1
(Serum containing cryopreservation medium)
Description:
CELLBANKER® 1 is a ready-to-use cell cryopreservation medium with serum containing formulation for broad
spectrum of mammalian cell cultures.The unique formulation allows for stable cryopreservation and high viability
after freeze-thaw procedure, even for sensitive cell lines.
Package:
100ml/bottle
20ml/bottle
CELLBANKER® 2 细胞冻存液
CELLBANKER® 2
(Serum-free cryopreservation medium)
Description:
CELLBANKER® 2 is a serum-free cell cryopreservation medium with unique complete formulation for broad
spectrum of mammalian cell cultures.
The unique formulation allows for stable cryopreservation and high viability after freeze-thaw procedure, without
potential risk ofcontamination serum.Optimum for serum-free cultured cells and peptide/protein expressing cells
cryopreservation.
Package
100ml/bottle
20ml/bottle
CELLOTION® 细胞冻存液
CELLOTION®
(Cell wash and recovery solution)
Description:
CELLOTION® is a chemically defined cell washing solution helps to increase the yield of cell recovery.
The unique formulation free of serum, protein and sugars,enables for cells, tissues and organs washing.
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文献和实验科幻电影中将冻存若干年的生命体重新解冻复活的情节在生命科学研究过程中每一天都在上演。为了将每一种有生命的细胞较好的保存其原有的细胞特性或者长久的保存种质资源，实验人员往往将细胞用特殊配置的细胞冻存液保存于-196℃ 的液氮，使得细胞暂时脱离生长状态，等到实验需要的时候再从液氮中取出复苏应用。在这一看似神奇的生命存储过程中，我们不禁要问，这是如何实现的呢？ 其实，细胞冻存和复苏的教科书和实验手册上面都有。小编不想再重复了。这里，我要谈谈应该注意的一些事项和技巧，大部分都是血泪教训
流式课堂 | 裂红 or 分离液？外周血不同制备方法应用场景
、粒细胞等），需要裂解红细胞；倘若检测的是红细胞，不需要裂解红细胞； ⊙ 如果后续样本不用于流式分析，而是需要培养或者冻存复苏，使用裂红后培养的细胞，活性可能会打折扣。 Ficoll 密度梯度离心法 01 原理 根据细胞的沉降系数差异，借助细胞分离液和离心操作，对细胞进行分离。 02 优点 ⊙ 获得的细胞更纯净，有利于细胞培养后检测胞内因子 ⊙ 可将细胞冻存后再复苏、培养、检测 ⊙ 去除死细胞 03 缺点 ⊙ 步骤复杂，需 30min 以上 ⊙ 取白膜层需要丰富的经验 ⊙ 只能获得淋巴细胞和单核
运输，收到后立即转入液氮或者-80 度冰箱冻存或直接复苏；（2）存活细胞，收到后应继续生长，传代达到细胞生长状态良好时，再进行冻存。具体操作见细胞培养步骤。收到细胞后请拍照，3 天内如果发现污染，请及时拍照与我们联系。 四、细细胞接收后的处理 1）收到细胞后，请检查发货培养瓶的状况，若发现培养瓶破损、有液溢出及细胞有污染，请拍照后及时联系我们。 2）在显微镜下确认细胞生长状态时，最好在低倍镜（4 或 5X 物镜)下进行，能准确判断细胞的传代密度。看细胞的形态请在 10X 和 20×物镜下，同时给刚收到
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