相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冰袋
- 保质期:
12个月
- 规格:
50Tests/100Tests/100Tests×2
| 规格: | 50Tests | 产品价格: | ¥461.0 |
|---|---|---|---|
| 规格: | 100Tests | 产品价格: | ¥691.0 |
| 规格: | 100Tests×2 | 产品价格: | ¥3300.0 |
| Alternate Names |
IL-2 receptor subunit alpha;IL-2-RA;IL-2R subunit alpha;IL2-RA;IL2RA;Interleukin-2 receptor subunit alpha;TAC antigen;p55
|
| Swissprot |
P01590
|
| Gene ID |
16184
|
| Clone No |
PC-61.5.3
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG1, κ
|
| Isotype Control |
Elab Fluor®Violet 610 Rat IgG1, κ Isotype Control[HRPN]
|
| Conjugation |
Elab Fluor®Violet 610
|
| Conjugation Information |
Elab Fluor®Violet 610 is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 613 nm (e.g., a 615/20 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|

| E-AB-F11020 | AF/LE Purified Anti-Mouse CD25 Antibody[PC-61.5.3] | 500μg , 50μg , 1mg |
| E-AB-F1102A | Purified Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102B | Biotin Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102C | FITC Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102D | PE Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102E | APC Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102G | PE/Cyanine5 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102H | PE/Cyanine7 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102I | PE/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102J | PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102L | Elab Fluor® 488 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102M | Elab Fluor® 647 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102P | PE/Elab Fluor® 594 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102Q | Elab Fluor® Violet 450 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102S | Elab Fluor® Red 780 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100Tests , 50Tests , 100Tests×2 |
| E-AB-F1102UC | FITC Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UD | PE Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UE | APC Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UG | PE/Cyanine5 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UH | PE/Cyanine7 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UI | PE/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UJ | PerCP/Cyanine5.5 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UL | Elab Fluor® 488 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UM | Elab Fluor® 647 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UP | PE/Elab Fluor® 594 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102UQ | Elab Fluor® Violet 450 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
| E-AB-F1102US | Elab Fluor® Red 780 Anti-Mouse CD25 Antibody[PC-61.5.3] | 100μg , 25μg |
Q1:一抗的使用浓度是多少?
我们的抗体是浓缩型的,抗体最终使用浓度除了跟抗体本身浓度有关,也跟样本中目的蛋白的丰度有关,所以我们说明书提供的抗体推荐稀释比是一个范围,最终稀释比例是需要您通过预实验确定的,当然您也可以咨询我们的技术团队给您推荐一个合适的稀释比来摸索实验条件。
Q2:IHC实验,想知道抗原修复条件是什么?是用EDTA还是枸橼酸钾?
修复条件一般为高温高压或者微波加热修复。 例如:抗原修复(高压法) :压力锅中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液(pH 6.0) (配制:将抗原修复液溶于相应体积 ddH2O中,混匀),加热至沸腾,切片置耐热塑料切片架上,放入锅中,盖好锅盖,扣上压力阀,继续加热,设置保压 4 min,时间到后打开放气阀放气,压力归零后开锅盖将内锅取出放置室温冷却,待溶液冷却至室温后取出切片(约30 min)。 抗原修复(微波法) :烧杯中加入足以淹没切片的 10 mmol/ml 的枸橼酸盐缓冲液(pH 6.0) (配制:将抗原修复液溶于相应体积 ddH2O中,混匀),加热至沸腾,切片置耐热塑料切片架上,放入烧杯中,中小火加热30min后取出,放置室温冷却,待溶液冷却至室温后取出切片(约30 min)。 IHC修复液的选择建议参考抗体使用说明书,或者参考以下方案:人体样本,优先推荐使用pH9.0的EDTA修复液(临床样本);大/小鼠样本,优先推荐使用pH6.0的枸橼酸修复液(科研样本)。
Q3:有IHC实验应用性的抗体,20μl可以做几张片子?
首先客户需要根据抗体说明书&预实验结果来预估一下抗体的使用量,不同的样本,因抗原的表达量不同,抗体稀释比会有差异的。 例如:某个抗体的IHC实验,预实验确认按稀释比1:50计算,通常情况下,一张切片需要加入稀释后的抗体100μl,也就是一张切片需要2μl抗体,20μl大概可以做10张切片。
Q4:IHC在数据表上是什么意思?是冷冻切片吗?与IHC-F区别?
IHC是免疫组化实验,使用的切片一般包含有IHC-P(石蜡切片)和IHC-F(冰冻切片)两种。
Q5:文献查的分子量与说明书上的有异议,同一指标为何有几种分子量?
因为同一蛋白在不同的种类的细胞中可能由于转录后有不同的剪切体、翻译后有不同的修饰方式,比如糖基化、泛素化等,分子量并不是唯一不变的,另外,蛋白官网Uniprot上可以查找到蛋白不同的亚型分子量大小是有差异的。所以文献上查到的分子量与说明书上抗体分子量有些会有一定的差异。(可以多查一些文献参考确认目的蛋白在目标样本中的分子量)。
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
. 2. Combining AbC™ anti-Rat/Hamster and ArC™ Amine Reactive Compensation Bead Kits The ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) is designed to facilitate compensation when using any of the LIVE/DEAD® fixable dead cell stains
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
技术资料暂无技术资料 索取技术资料







![Elab Fluor®Violet500 Anti-MouseCD16/32 Antibody[2.4G2]](https://img1.dxycdn.com/p/s14/2024/1021/959/2323667303219828481.jpg!wh200)
