相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
31
- 英文名:
Ionomycin free acid
- CAS号:
56092-81-0
- 供应商:
上海莼试
- 保存条件:
Desiccate at -20°C
- 规格:
1mg
| 货号 | CS-01Y65704 | 规格 | 1mg |
| CAS号 | 56092-81-0 | 分子量 | 709.01 |
| 含量 | >98.00% | 别名 | N/A |
| 分子式 | C41H72O9 | 化学名 | (4R,6R,8R,10E,12S,14S,16E,18S,19S,20R,21S)-11,19,21-trihydroxy-22-((2R,2'R,5R,5'R)-5'-((R)-1-hydroxyethyl)-2,5'-dimethyloctahydro-[2,2'-bifuran]-5-yl)-4,6,8,12,14,18,20-heptamethyl-9-oxodocosa-10,16-dienoic acid |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C41H72O9

分子量:709.01
溶解度:1.4mg/mL in DMSO, 2.5mg/mL in DMF
储存条件:Desiccate at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Ionomycin is a selective and potent calcium ionophore, which acts as a motile Ca2+ carrier.by direct stimulation of store-regulated cation entry across biological membranes, ionomycin can effectively enhance Ca2+ influx.In vitro: Calcium ion can be extracted by ionomycin fom the queous phase into the organic phase. Ionomycin also acts as a mobile ion carrier, which transports the cation across a solvent barrier [1].In vivo: Efficiency of ionomycin on oocyte activation and suquent development was evaluated, which identified ionomycin as an efficient activator at the concentation of 10 μmol/l. With a second exposure to 5 μmol/l ionomycin on blastocyst development, an improved effect was found. No adverse effects of ionomycin on mouse embryo development were identified [2].Clinical trial: One randomized, prospective, controlled study has been performed to analyze the effect of calcium ionophore solution on fertilization rate in patients with diminished ovarian reserve, which did not orve any differences in fertilization, clinical pregnancy, or ongoing pregnancy rates between the groups [3].
注意事项:
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
使用方法:
1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
公司正在出售的产品:
| Recombnant Human ARPC5L | Cy5标记的小鼠抗牛IgG H&L |
| APC标记的驴抗羊IgG H&L | Donkey Anti-Goat IgG H&L / Cy5 |
| 罗丹明标记的兔抗豚鼠IgM | Mouse Anti-Goat IgG H&L / FITC |
| PE-CY5标记的驴抗兔IgG H&L | Rabbit Anti-Mouse IgM / Cy3 |
| Alexa Fluor 350标记的羊抗兔IgG H&L | Goat Anti-Mouse IgG H&L / AF750 |
| PE-Cy7标记的羊抗兔IgG H&L | Rabbit Anti-Horse IgG H&L / AP |
| 罗丹明标记的兔抗小鼠k链 | Mouse Anti-Rat IgG H&L / AP |
| Cy5标记的羊抗鸡IgG H&L | 端粒酶抑制因子PnX-1封闭多肽 |
| Cy5标记的驴抗羊IgG H&L | Goat Anti-Chicken IgG H&L / Cy5 |
| FITC标记的小鼠抗羊IgG H&L | Rabbit Anti-Mouse Kaa light chain / RBITC |
| Alexa Fluor 750标记的羊抗小鼠IgG H&L | Goat Anti-Rabbit IgG H&L / PE-Cy7 |
| Cy3标记的兔抗小鼠IgM | Goat Anti-Rabbit IgG H&L / AF350 |
| 碱性磷酸酶(AP)标记的兔抗马IgG H&L | Ionomycin free acidDonkey Anti-Rabbit IgG H&L / PE-Cy5 |
| 碱性磷酸酶(AP)标记的小鼠抗大鼠IgG H&L | Rabbit Anti-Guinea Pig IgM / RBITC |
| Mouse Anti-Bovine IgG H&L / Cy5 | Donkey Anti-Goat IgG H&L / APC |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验proteinogenic/free amino acids or central carbon metabolites, and (2) a mathematical model that relates the unknown fluxes to the measured isotopomers. Modeling requires a thorough knowledge of the structure of the underlying metabolic network, often available
NOTE - Use arachidonic acid from Biomol (# FA-003) 1) Make up the appropriate concentration of AA in 100% ethanol. 2) Add 1µl of phenol red solution. --> The solution should appear yellow since the free acid
1) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum. 2) Pellet cells and wash 1 time with room temperature PBS. 3) Resuspend final pellet in an appropriate volume of prewarmed serum free media and supplement
技术资料暂无技术资料 索取技术资料









