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- 详细信息
- 文献和实验
- 技术资料
- 库存:
28
- 英文名:
croside II
- CAS号:
39012-20-9
- 供应商:
上海莼试
- 保存条件:
4°C, protect from light
- 规格:
0mM (in 1mL DMSO) 20mg
| 货号 | CS-01Y65939 | 规格 | 0mM (in 1mL DMSO) 20mg |
| CAS号 | 39012-20-9 | 分子量 | 512.46 |
| 含量 | >98.00% | 别名 | 6-Vanilloylcatalpol |
| 分子式 | C23H28O13 | 化学名 | [1a-(hydroxymethyl)-2-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,5a,6,6a-tetrahydro-1bH-oxireno[5,6]cyclopenta[1,3-c]pyran-6-yl] 4-hydroxy-3-methoxybenzoate |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C23H28O13

分子量:512.46
溶解度:≥ 22.9mg/mL in DMSO
储存条件:4°C, protect from light
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
PicrosideII is a major an iridoid glucoside isolated from Picrorhiza scrophulariiflora Pennell (Scrophulariaceae). Previous studies have shown that picroside II can protect cardiomyocytes against oxidative stress induced by hypoxia/ reoxygenation (H/R) injury.In vitro: Pretreated cardiomyocytes with picroside II was found to inhibit LDH activity in culture media and increase cell viability in a dose-dependent manner. Such protective effect was accompanied with significant reduction of GSH contents and the activities of SOD and GSH-Px. Attenuated MDA and GG contents in response to H/R injury were orved as well. In addition, picroside II treatment also inhibited calcium accumulation and ROS production in cardiomyocytes. [1].In vivo: A rat focal cerebral ischemic model was established, and picroside II were given intraperitoneally. Results indicated that the damage to the structures of the neurons and the blood brain barrier (BBB) in the cortex was more severe in the model group. In the picroside II treatment group, the neurological function, the morphology and ultrastructure of the neurons, and the BBB were improved. In addition, apoptotic cell number, cerebral infarct volume, EAR and pERK1/2 expression were decreased significantly compared to the model group [2].
注意事项:
抗逆滴加序列
每次向板内滴加抗原时,移液器滴头要与平面45度悬空,不要触碰到孔内的液体,由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下,必须反应30min以上,若环境温度低于室温,可将微量反应板置于恒温培养箱中,使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定,往往在高压后pH值会有所改变,所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时,红细胞会产生自凝现象;当pH>7.8时,图形洗脱加快,易造成肉眼观察产生误差;p H=7.2时,红细胞沉降最充分,图形最清晰。
当滴注 1%红细胞悬液时,应经常摇晃红细胞悬液,使红细胞均匀地分布在磷酸缓冲液中,以防止红细胞下降。
反应时间及温度
加入鸡红血球之后,反应板在室温(20~25℃)静置30~40min,对照孔血球下沉于孔底,即可判定结果。若室温达不到实验要求,需相应调整反应时间。当环境温度低于4℃时,红细胞发生自凝;高于37℃时,会发生反应物分离和红细胞溶血。
使用方法:
1.本蛋白酶抑制剂混合物为100×的储存液,使用时按照1:100的比例加入到裂解液中(例如,1ml裂解液中加入10μl蛋白酶抑制剂混合物),混匀后即可使用。根据需要,0.5M的EDTA也按照1:100的比例加入到裂解液中(如用于检测金属蛋白酶活性,则不宜添加EDTA)。含有蛋白酶抑制剂混合物的裂解液宜现用现配,不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后,就可以开始进行哺乳动物组织的裂解和蛋白提取。
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文献和实验主要存在于线粒体中的一类内含子,它的剪接位点类似于核编码结构基因的内含子,并同样遵从GT--AG规律。剪接机理同核内含子的剪接相似,也要形成一个套索的中间体,通过形成5'-2'磷酸二酯键将要剪接的位点靠近到一起。但是,II型内含子的剪接又不完全与核内含子的剪接相同,它具有自我剪接的功能,不需要剪接体和snRNA的参与,也不需要ATP供能。从结构上看,II型内含子的6个结构域可形成发夹环, 结构域5与6之间只间隔3个碱基,结构域6参与转酯作用。
Ubiquitinated protein detection is often troublesome since in most cases this modification reduces the half-life of targeted proteins, inducing their degradation. Furthermore, ubiquitination is reversible thanks to the action of highly
Characterization of Angiotensin II Receptors
Abstract Table of Contents Materials Figures Literature Cited Abstract Angiotensin II (Ang
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