- ￥1380 - 2080
- 艾普蒂
- 武汉
- PA1000-9545
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C/-80°C upon receipt
- 保质期：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名：
Epidermal growth factor receptor kinase substrate 8-like protein 1
- 库存：
50支
- 供应商：
艾普蒂
- 规格：
20μg/50μg
| 规格： | 20μg | 产品价格： | ￥1380.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥2080.0 |
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文献和实验Human Embryonic Stem (ES) Cell Protocols――Thawing Human ES cells
Remove Human ES cells from liquid nitrogen storage tank. Fill out a freeze/thaw form. Thaw cryovial by gently swirling in waterbath until only a small ice pellet remains, being careful not to completely submerge the cryovial under water.
Human Embryonic Stem (ES) Cell Protocols――Freezing Human ES Cells
pellet (gently) in 3 ml ES media to wash away collagenase. Spin 5 minutes at 1000 rpm. Resuspend pellet (again, gently!) in 0.25 ml Resuspension Medium per vial (This is one half the final volume required for freezing). Dropwise, add
Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on MEFs
collagenase off and wash cells with 3 ml human ES media. Spin 1000rpm 5 minutes. While the cells are spinning prepare the feeder layers by aspirating off the MEF media and washing one time with Ca/Mg free PBS and adding human ES media to the feeder
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