- ￥600 - 3200
- 上海莼试
- CS-01Y69190
- 国产
- 2025年07月12日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
22
- 英文名：
L-Asparagine (hydrate)
- CAS号：
5794-13-8
- 供应商：
上海莼试
- 保存条件：
-20°C
- 规格：
1 g 5 g 10 g
分子式:C4H8N2O3·H2O
分子量:150.1
溶解度:Methanol: slightly soluble,PBS (pH 7.2): slightly soluble
储存条件:-20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
L-Asparagine is a non-essential amino acid.1 It is formed from L-aspartic acid and L-glutamine by asparagine synthetase (ASNS), and it is deamidated by L-asparaginase to produce L-aspartic acid and ammonia.2,3 L-Asparagine (0.3 mM) reverses ASNS siRNA knockdown-induced decreases in proliferation in a panel of six human cancer cell lines, indicating that both exogenous and endogenous L-asparagine promote proliferation of these cells.4 Formulations containing L-asparagine have been used as dietary supplements.1.Chiu, M.I., Taurino, G., Bianchi, M.G., et al.Asparagine synthetase in cancer: Beyond acute lymphoblastic leukemiaFront. Oncol.91480(2020) 2.Zhu, W., Radadiya, A., Bisson, C., et al.High-resolution crystal structure of human asparagine synthetase enables analysis of inhibitor binding and selectivityCommun. Biol.2345(2019) 3.Covini, D., Tardito, S., Bussolati, O., et al.Expanding targets for a metabolic therapy of cancer: L-AsparaginaseRecent Pat. Anticancer Drug Discov.7(1)4-13(2012) 4.Pathria, G., Lee, J.S., Hasnis, E., et al.Translational reprogramming marks adaptation to asparagine restriction in cancerNat. Cell Biol.21(12)1590-1603(2019)
商品属性：
| 货号 | CS-01Y69190 | 规格 | 1 g 5 g 10 g |
| CAS号 | 5794-13-8 | 分子量 | 150.1 |
| 含量 | >99.00% | 别名 | N/A |
| 分子式 | C4H8N2O3·H2O | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
| Recombnant Human CARB2 proten ,C- H Tag | GPX1 Antibody Blocking Peptide |
| Recombinant Human Borealin | Recombinant Mouse Mup20 |
| HLN1 Antibody Blocking Peptide | Recombinant Mouse MUSK |
| HRC Antibody Blocking Peptide | Recombinant Mouse Myoglobin |
| RERE Antibody Blocking Peptide | Recombinant Mouse MYL9 |
| phospho-CSNK1A1 (Tyr294) Antibody Blocking Peptide | Recombinant Mouse NICE2 |
| CAB39 Antibody Blocking Peptide | Recombinant Mouse Nicotinic Acetylcholine Receptor alpha 1 |
| GRHL3 Antibody Blocking Peptide | 磷酸化活化转录因子4封闭多肽 |
| FGF21 Antibody Blocking Peptide | Recombinant Mouse MRP8 |
| GLT8D1 Antibody Blocking Peptide | Recombinant Mouse MIP-3 beta |
| Recombinant HCoV-NL63 protein, His | Recombinant Mouse MIF |
| ZC3H13 Antibody Blocking Peptide | Recombinant Mouse Midkine |
| M-ras Antibody Blocking Peptide | L-Asparagine (hydrate)Recombinant Mouse METRNL |
| MUSK Antibody Blocking Peptide | Recombinant Mouse MESDC2 |
| NPM2 Antibody Blocking Peptide | Recombinant Mouse MDH2 |
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
utilizes the determination of ammonia liberated from L-asparagine in the enzyme reaction by the Nessler reaction. L-asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. Start the reaction by adding 0.1 ml
Extraction of DNA from Schizophyllum commune （裂褶菌DNA提取）
and Thompson. Nucleic Acids Res. 8:4321-4325; 1980.) 0.1 P agar For 1 L total medium: 5 ml Phosphate stock 1 ml trace elements stock 5 ml thiamine HCl 1 g L-asparagine 0.5 g MgSO4 20 g Glucose 10 g Agar 0.002 P liquid medium For 1 L total medium
Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase
. Prepare cell suspensions also in distilled water or 0.05 M KPi with no hexane as controls. Measure the enzyme activity utilizing the Nesslerization reaction. This method utilizes the determination of ammonia liberated from L-asparagine
技术资料暂无技术资料 索取技术资料
