- ￥180
- Agarose, Regular
- 2025年08月11日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
A620014-0100
概述
琼脂糖是从琼脂或含琼脂海藻中提取的聚合物。由交替的共聚物D-半乳糖和3,6-脱水-L-半乳糖单元通过糖苷键连接组成，具有出色的生物相容性、机械特性及扩散特性。琼脂糖分离范围广，简便，准备方便，不需要催化剂。本品为标准熔点琼脂糖，专为常规分离分析而设计。使用其制备的琼脂糖凝胶电泳，可分离不同分子量范围内的DNA和RNA 片段。
应用
广泛用于生物学、免疫学、生物化学和微生物学研究。可作为用于常规核酸电泳实验、Southern Blot、Northern Blot、凝胶剂、用于脂蛋白和免疫电泳等。
属性
| 凝胶强度 | ≥1200 g/cm^2 (1% Gel) |
| 凝胶温度 | 35~37°C (1.5% Gel) |
| 溶胶温度 | 87~89°C (1.5% Gel) |
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文献和实验ELECTROPHORESIS OF DNA IN AGAROSE GELS
fragments, and 1 - 1.2% for smaller DNA fragments. 0.5% gels are very flimsy and need to be handled with caution. Low melting agarose is used at the same concentration as regular agarose. We have used FMC's SeaPlaque agarose which melts at 65°C
(even if you're not going to recover the insert by melting the gel slice)-- the regular agarose supposedly has more of the “something” that inhibits ligase.Since low melt agarose is “sloppier” than regular agarose,you will probably have to run a 1.5% or 2.0% gel for the gel
the gel slice)-- the regular agarose supposedly has more of the “something” that inhibits ligase.Since low melt agarose is “sloppier” than regular agarose,you will probably have to run a 1.5% or 2.0% gel for the gel separation.
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