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- TD-AB0027
- 2025年11月25日
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- 技术资料
- 供应商：
武汉天德
- 库存：
999
- 级别：
科研级
- 抗体英文名：
Anti-VIM mouse monoclonal antibody
- 抗体名：
Vimentin
- 规格：
25 μl/100 μl/200 μl
Vimentin mouse monoclonal antibody
| Product Name | Vimentin mouse monoclonal antibody |
| Cat.# | TD-AB0027 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | Anti-VIM mouse monoclonal antibody |
| Applications | WB |
| Immunogen | Fusion protein of human VIM |
| Reactivity | Human, Mouse |
| Host species | Mouse |
| Ig class | IgG1 |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | VIM |
| Synonyms | Vimentin |
| Target Background | |
| This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. | |
| Western blotting | |
| Predicted band size | 54 kDa |
| Positive control | HUVEC, 293T, NIH/3T3 and Hela cell lysates |
| Recommended dilution | 500-2000 |
|
Product Image | |
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
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