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- TD-AB0022
- 2025年11月25日
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- 技术资料
- 供应商：
武汉天德
- 库存：
999
- 级别：
科研级
- 抗体英文名：
V-akt murine thymoma viral oncogene homolog 1
- 抗体名：
AKT1
- 规格：
25 μl/100 μl/200 μl
AKT1 mouse monoclonal antibody
| Product Name | AKT1 mouse monoclonal antibody |
| Cat.# | TD-AB0022 |
| Package | 25 μl/100 μl/200 μl |
| Storage | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
| Product Imformation | |
| Description | Anti-AKT1 mouse monoclonal antibody |
| Applications | WB |
| Immunogen | Fusion protein of human AKT1 |
| Reactivity | Human |
| Host species | Mouse |
| Ig class | IgG1 |
| Purity | Protein A/G Affinity Purification |
| Target information | |
| Symbol | AKT1 |
| Full name | V-akt murine thymoma viral oncogene homolog 1 |
| Target Background | |
| The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. | |
| Western blotting | |
| Predicted band size | 56 kDa |
| Positive control | Hela, 231, PC-3 and HepG2 cell lysates |
| Recommended dilution | 1: 1000-5000 |
| Gel | 8%SDS-PAGE |
| Lysate | 40 μg |
| Lane 1-4 | Hela, 231, PC-3 and HepG2 cell lysates |
| Primary antibody | D191053(AKT1 Antibody) at dilution 1/1200 |
| Secondary antibody | Goat anti mouse IgG at 1/5000 dilution |
| Exposure time | 2 minutes |
| Product Image | |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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