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Trichomonas spp.滴虫（毛滴虫）染料法荧光定量PCR试剂盒上海圻明生物优势供应。更多产品资料欢迎免费咨询。
One of the many important uses of PCR technology is that it can be used to label DNA probes with high specific activity. PCR technology has high specificity, and can synthesize probe DNA fragments in quantities within 1~2h if [α-32P]dNTP or other markers are added to the substrate
dNTPs, the probe DNA can be well labeled during DNA synthesis, and the incorporation rate of the marker can be as high as 70%~80%. Therefore, PCR labeling technology is particularly suitable for large-scale detection and non-radiolabeling. The disadvantage of this method is that a specific pair of PCR primers is synthesized.
Labeling can also be achieved by using small fragments prepared from probe DNA as primers.
Solution preparation
1. Prepare a stock solution
Unless otherwise stated, all unused stock solutions should be divided into disposable aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
1.1* Acid Stock Solution (125X):
Add 20 μL DMSO to *ate (component B) to make a 125X* acid stock solution.
2. Prepare standard solutions
*Salt standard solution
Add 50 μL of 1 mM KH2PO4 (Component C) to 950 μL of deionized water or enzyme reaction buffer to give a 50 μM * saline standard solution (PS7). A 50 μM * saline standard solution (PS7) was taken and serially diluted 1:2 to obtain a serially diluted phosphate standard with deionized water or enzyme reaction buffer.
3. Prepare a working solution
Add 20 μL of 125X* stock solution to 2.5 mL of sterile H2O and mix well to make a working solution of *salt. Avoid potential Pi contamination. Note: Avoid direct exposure of *salts (component B) to light. Due to the high sensitivity of this assay to Pi, it is extremely important to use Pi-free labware and reagents.
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文献和实验佚名 阴道毛滴虫（Trichomonas vaginalis Donne1837）是寄生在人体阴道及泌尿道的鞭毛虫，主要引起滴虫性 阴道炎 ，是以性传播为主的一种传染病，全球性分布，人群感染较普遍。 一、形态 阴道毛滴虫
寄生在人阴道内，体呈梨形，前端有4条鞭毛，波动膜短，附着在膜外缘的鞭毛不游离出体外。此虫寄生可引起阴道炎。 寄生在鱼鳃的鳃隐鞭毛虫（Cryptobia branchialis），体叶状，具2鞭毛，1向前伸，1沿波动膜向后伸出，游离出体外，似尾状，可致鱼死亡。有的鞭毛虫具有极多的鞭毛，如寄生在白蚁肠内的披发虫（Trichonympha），白蚁能消化木材中的木质纤维，就是靠披发虫的作用。如以高温将白蚁肠内的披发虫杀死，而不伤白蚁，白蚁再食木材后，即不能消化而饿死。
佚名 阴道毛滴虫（Trichomonas vaginalis Donne1837）是寄生在人体阴道及泌尿道的鞭毛虫，主要引起滴虫性 阴道炎 ，是以性传播为主的一种传染病，全球性分布，人群感染较普遍。 一、形态
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