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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Immortal rat small intestinal smooth muscle cells
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
NO
- 细胞类型：
永生化
- ATCC Number：
11222
- 品系：
SD大鼠
- 组织来源：
小肠组织
- 相关疾病：
无
- 物种来源：
大鼠
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
小肠
- 运输方式：
常温
- 年限：
5年
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化大鼠小肠平滑肌细胞简介：
产品描述：大鼠小肠平滑肌细胞分离自新生大鼠小肠组织。小肠位于腹中，上端接幽门与胃相通，下端通过阑门与大肠相连，是食物消化吸收的主要场所。小肠有三种功能即消化、吸收和分泌及运动功能，其中以吸收和分泌功能为主。平滑肌细胞的收缩是负责肠蠕动的动力，促使食物向下运动。
产品货号：IR3009
产品类型: 永生化细胞
传代能力: 30代左右
产品形态: 梭形
培养基：永生化大鼠小肠平滑肌细胞完全培养基
支原体：呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：1周左右货期
永生化大鼠小肠平滑肌细胞白光图
Immortalization of primary rat cells by human papillomavirus type 16 subgenomic DNA fragments controlled by the SV40 promoter
We tested the human papillomavirus 16 (HPV 16) early genes for their ability to immortalize or transform primary rat brain cells, using the expression plasmids that contain the neomycin-resistance-inducing unit (pSV2neo) and the transcriptional unit for the HPV 16 subgenomic DNA fragments controlled by the SV40 early promoter. After transfection, drug-resistant colonies were maintained by refeeding and replating for characterization. The E7 gene alone was found to be capable of immortalizing and morphologically transforming primary rat cells, and the transformed cells showed anchorage-independent growth. Although its activity was lower than that of the E7 gene, the E6 gene also immortalized primary rat cells. The immortalized or transformed cells contained HPV 16-specific DNA and mRNA.
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
胚胎成纤维细胞 NBLE 新生牛眼晶体上皮细胞 NBTF 新生牛眼Tenon's囊成纤维细胞 NIH3T3 小鼠胚胎成纤维细胞 P815 小鼠肥大细胞 PA12 小鼠成纤维细胞 RCBBF 兔角膜后基质层成纤维细胞 RCFBF 兔角膜前基质层成纤维细胞 RTE 大鼠气管上皮细胞 RYTF 兔眼Tenon's囊成纤维细胞 SF9 昆虫卵巢细胞 SMC 兔主动脉平滑肌细胞 Vero 猴肾细胞 WEHI-3 小鼠血液细胞 Y2
3K的下游靶点。 刚刚接触这方面的信号通路，望多指教，谢谢！ yhwangcams 呵呵，好多情况下得出的结论都可能源于不同的细胞系，不同的处理方法，等等。 各个信号通路间会有一些“交流”（crosstalk）。我的建议是先从Nature, Cell等上找一些大人物写的综述看看。基本了解后，再看相关细胞里（如平滑肌细胞等）的研究。自己能做出什么结果，只要可信，就可以得出相关结论。 本文由丁香园论坛提供，想了解更多有用的、有意
主要有两类：神经嵴干细胞(neuralcreststemcell，NC-SC)和中枢神经干细胞(CNS-SC)。NCSC为外周神经干细胞(PNS-SC)，既可发育为外周神经细胞、神经内分泌细胞和Schwann氏细胞，也能分化为色素细胞(pigmented cell)和平滑肌细胞等。NSC一般是指存在于脑部的中枢神经干细胞(CNS-SC)，其子代细胞能分化成为神经系统的大部分细胞。以往认为，中枢神经系统的神经元在出生前或出生后不久，就失去再生能力。但近年的一些研究表明，成年哺乳
技术资料