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- 详细信息
- 文献和实验
- 技术资料
- 库存：
45
- 英文名：
Teicoplanin A3-1
- CAS号：
93616-27-4
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
1mg 5mg
使用方法：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
产品描述：
分子式:C72H68Cl2N8O28
分子量:1564.3
溶解度:Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Teicoplanin A3-1, also known as Antibiotic L 17054, is a glycopeptide antibiotic derived from Actinoplanes teichomyceticus and produces a potent broad spectrum antibiotic activity against gram-positive bacteria.Teicoplanin A3-1 is a common degradation product of teicoplanins A2-1 to 5, which results from cleavage of the lipoaminoglycoside substituents. Teicoplanins are glycopeptide antibiotics produced by A. teichomyceticus which are effective against gram-positive bacteria, including methicillin-resistant Staphylococcus aureu (MRSA) and E. faecalis. As an antibiotic complex, teicoplanin consists of five closely related factors, T-A2-1, 2, 3, 4 and 5 and a more polar factor, T-A3-1. Additionally, as a glycopeptide antibiotic belonging to the same family as vancomycin, teicoplanin blocks cell wall synthesis in Bacillus subtilis, which is accompanied by an intracellular accumulation of UDP-N-acetylmuramyl-pentapetide [1]. Due to the greater antibacterial activity of teicoplanin than other agents, it has been thoroughly studied as a β-1actamase-resistant agent for the treatment of human infections caused by streptococci and staphylococci, including enterococci [2].In vitro: Up to now, in vitro study of Teicoplanin A3-1 is still in the development stage.In vivo: Up to now, in vivo study of Teicoplanin A3-1 is still in the development stage.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
| 货号 | CS-01Y72838 | 规格 | 1mg 5mg |
| CAS号 | 93616-27-4 | 分子量 | 1564.3 |
| 含量 | >98.00% | 别名 | Antibiotic L 17054 |
| 分子式 | C72H68Cl2N8O28 | 化学名 | 34-O-[2-(acetylamino)-2-deoxy-β-D-glucopyranosyl]-22,31-dichloro-7-demethyl-64-O-demethyl-19-deoxy-42-O-α-D-mannopyranosyl-ristomycin A aglycone |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C72H68Cl2N8O28
分子量:1564.3
溶解度:Soluble in ethanol;Soluble in methanol;Soluble in DMSO;Soluble in dimethyl formamide
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Teicoplanin A3-1, also known as Antibiotic L 17054, is a glycopeptide antibiotic derived from Actinoplanes teichomyceticus and produces a potent broad spectrum antibiotic activity against gram-positive bacteria.Teicoplanin A3-1 is a common degradation product of teicoplanins A2-1 to 5, which results from cleavage of the lipoaminoglycoside substituents. Teicoplanins are glycopeptide antibiotics produced by A. teichomyceticus which are effective against gram-positive bacteria, including methicillin-resistant Staphylococcus aureu (MRSA) and E. faecalis. As an antibiotic complex, teicoplanin consists of five closely related factors, T-A2-1, 2, 3, 4 and 5 and a more polar factor, T-A3-1. Additionally, as a glycopeptide antibiotic belonging to the same family as vancomycin, teicoplanin blocks cell wall synthesis in Bacillus subtilis, which is accompanied by an intracellular accumulation of UDP-N-acetylmuramyl-pentapetide [1]. Due to the greater antibacterial activity of teicoplanin than other agents, it has been thoroughly studied as a β-1actamase-resistant agent for the treatment of human infections caused by streptococci and staphylococci, including enterococci [2].In vitro: Up to now, in vitro study of Teicoplanin A3-1 is still in the development stage.In vivo: Up to now, in vivo study of Teicoplanin A3-1 is still in the development stage.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
公司正在出售的产品：
| Recombnant Human LAMP2 proten | Recombnant Human TOM1L2 |
| Recombnant Human TOMM34 | 氨甲酰磷酸合成酶1封闭多肽 |
| Recombnant Human Torn A | 甲基化样蛋白7B封闭多肽 |
| Recombnant Human TOX4 | CD53封闭多肽 |
| Recombnant Human TPH2 | 长链脂肪酸延长酶长ELOVL6封闭多肽 |
| Recombnant Human TPK1 | 磷酸化三磷酸腺柠檬酸裂解酶封闭多肽 |
| Recombnant Human TPM4 | 转移相关蛋白MTA3封闭多肽 |
| Recombnant Human TPOR | PODN蛋白封闭多肽 |
| Recombnant Human TP | Wnt信号受体FZD2蛋白封闭多肽 |
| Recombnant Human TR-1 | 亲环素C封闭多肽 |
| Recombnant Human TRAF2 | 重组2 pke突变蛋白RBD(BQ.1.1/Omcron) |
| Recombnant Human TRAL | MORN5蛋白封闭多肽 |
| Recombnant Human Tranaldolae 1 | Teicoplanin A3-1雌激素受体α封闭多肽 |
| Recombnant Human Tranln | 染色质组装因子1P155封闭多肽 |
| FAM125A蛋白封闭多肽 | 细胞命运决定因子DACH1封闭多肽 |
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文献和实验相关实验
; they can be distinguished on the basis of the level and inducibility of resistance to vancomycin and teicoplanin.
On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis
of vancomycin (Van) from Streptomyces orientalis and teicoplanin (Teic) from Actinoplanes teicomyceticus to D-Ala-D-Ala terminus peptides.
法。 【关键词】 胶束电动毛细管色谱； 替考拉宁； 组分 ABSTRACT Objective To establish a micellar electrokinetic capillary chromatography method for separation of components of teicoplanin. Methods Concentration of surfactant, pH value, concentration of buffer
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Teicoplanin A3-1
￥600 - 3200
