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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Sesamum indicum Oleosin L
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Sesamum indicum Oleosin L品名简称:
Recombinant Sesamum indicum Oleosin L protein货号:
CSB-CF4861SYP规格:
20μg价格:
9720LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
N/AUniprot No.:
Q9XHP2别名:
(Allergen Ses i 5)(Oleosin 15 kDa)(allergen Ses i 5.0101)种属:
Sesamum indicum (Oriental sesame) (Sesamum orientale)蛋白长度:
Full Length来源:
in vitro E.coli expression system分子量:
16.7 kDa表达区域:
1-145aa氨基酸序列:
MAEHYGQQQQTRAPHLQLQPRAQRVVKAATAVTAGGSLLVLSGLTLAGTVIALTIATPLLVIFSPVLVPAVITIFLLGAGFLASGGFGVAALSVLSWIYRYLTGKHPPGADQLESAKTKLASKAREMKDRAEQFSQQPVAGSQTS蛋白标签:
N-terminal 10xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May have a structural role to stabilize the lipid body during desiccation of the seed by preventing coalescence of the oil. Probably interacts with both lipid and phospholipid moieties of lipid bodies. May also provide recognition signals for specific lipase anchorage in lipolysis during seedling growth.功能2:
/文献:
"Gene family of oleosin isoforms and their structural stabilization in sesame seed oil bodies." Tai S.S., Chen M.C., Peng C.C., Tzen J.T. Biosci. Biotechnol. Biochem. 66:2146-2153(2002)研究领域:
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文献和实验DNA包装完成以后,病毒衣壳变得致密,裂孔关闭,至少在衣壳内2/3处关闭。伴随病毒核酸包装,病毒衣壳膨胀,衣壳蛋白 构象可发生变化[8]。如噬菌体HK97为20面体立体对称结构,衣壳由415主要结构蛋白 构成,衣壳蛋白 亚基分子量为42KDa,无骨架蛋白 ,在病毒衣壳装配过程中可出现4种衣壳演变形成:①proheadⅠ:由415个42KDa的结构蛋白 亚基构成,衣壳蛋白 之间无酶解、共价交联和构象变化,易被离解为多个壳粒。②proheadⅡ:衣壳大小不变,由于衣壳蛋白 氨基端被水解而变为
Acute Asthma Models to Ovalbumin in the Mouse
. It comprises a sensitization step in the presence of aluminum hydroxide as an adjuvant, and a second step where mice are challenged with the allergen introduced directly into the airways to induce the modeled asthma features. This article describes procedures
。 ( 2 ) 为了得到高浓度的特异重组蛋白提取物(如在低拷贝质粒上重组蛋白质)用于随后的蛋白质纯化,我们可以同时用 100 μl 裂解液分别裂解两个相同的 1 ml 培养物的沉淀物,合并得到 200 μl 裂解物,供下一步分析 [15]。 ( 3 ) 小心吸取上清液,只将那些澄清的上清液转移到过滤板上,以确保在随后的过滤中板不会被堵塞。 ( 4 ) 使用适用于微孔板的真空泵。 ( 5 ) 确保在过滤后没有液体残留在过滤板下面;如果有的话,必须手动清除。为了避免这个问题的出现,过滤要在足够的真空
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