Phospho-CREB (Ser133) 多克隆抗体

Using Phospho‐Motif Antibodies to Determine Kinase Substrates

the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation

诺奖解读｜光遗传实现“光控大脑”，TR-FRET 试剂盒补齐神经信号定量短板

-FRET 试剂盒（p-CREB、p-ERK 等），可以定量光诱发神经放电之后翻译后修饰的变化水平，直接回答“神经元电活动如何转化为基因表达信号”这类机制问题。试剂盒基于双抗体夹心法，两个分别标记 Eu 和 FR 的抗体识别磷酸化位点与稳定区表位，不需要 WB 的制胶、电泳和曝光，两小时出结果。 THUNDER™ Phospho-CREB (S133) 检测数据：剂量依赖与 EC50 曲线 应用三：高通量化合物筛选 把表达 ChR2/optoXR 的稳定细胞株铺入微孔板，梯度加药、程序化光照刺激，再用

Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunocolocalization Using Phospho-Selective KOR, p38 MAPK, and KIR

), and causes activation of KOR. Agonist-activated KOR becomes a substrate for G protein receptor kinase (GRK), which phosphorylates the Ser369 residue at the C-terminal tail of the receptor in the first step in the β-Arrestin-dependent desensitization cascade