DNA-PK (Phospho Thr2609) rabbi

Phospho-Specific Antibodies as a Tool to Study In Vivo Regulation of BRCA1 After DNA Damage

Phospho-specific antibodies have become very useful reagents for study of signal transduction pathways. This chapter describes the production of phospho-specific antibodies and their use to assess individual phosphorylation events in vivo

Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein

The rabbit reticulocyte lysate (RRL) is a convenient system for in vitro translation of transcription factors (1 ). By using in vitro-synthesized transcription factors, the time-consuming and often difficult process of protein purification

Gene Delivery to Rabbit Arteries Using the Collar Model

delivery approaches (1 ). Plasmid DNA, transferred by an extravascular approach, gives transfection efficiency high enough to cause biological effects. Plasmids and adenoviruses can be used for both extravascular and intravascular gene therapy. The collar