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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询甄准
- 保质期：
咨询甄准
- 英文名：
3-Ethyl-4-methyl-3-pyrrolin-2-one
- 库存：
1
- 供应商：
上海甄准
- CAS号：
766-36-9
- 规格：
5g
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文献和实验tube mix: 15 µl dH2O (times number of samples + 1) 70 µl 1MTris Ph 8.0 (times number of samples + 1) 20 µl 4 mM Acetyl CoA (times number of samples + 1) and 5 µl 14 C CAM (times number of samples). 13. Add 0.5 ml Ethyl Acetate to each tube. 14
in 30 µl Ethyl Acetate. 19. Spot on silica gel TLC (in 5 µl amounts). 20. Develop TLC in 200 ml Chloroform/Methyl-OH (95:5) until solution is almost at the top (app. 1 hr.). 21. Dry & expose against film ON.
DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
and incubate at 37 C. The A culture is incubated for 30 minutes, the B culture is incubated for 60 minutes. Centrifuge for 6 minutes at 150 x g. For A culture go to Harvest Procedure step G. For B culture go to Harvest Procedure step D. Short-term: Pipet
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