CH033 rabbit pAb

Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates

All of the basic functional assays of complement activity utilize erythrocytes as targets (1 ). For classical pathway assays, the favored target is the antibody-sensitized sheep erythrocyte. For alternative pathway assays, unsensitized rabbit

噬菌体展示技术——高通量，有内涵

宿主菌的裂解，故称为噬菌体。噬菌体主要有双链噬菌体和单链丝状噬菌体两大类，双链噬菌体为λ类噬菌体，单链丝状噬菌体有M13、f1、fd噬菌体。   再聊聊噬菌体展示技术 噬菌体技术两大核心——建库和筛选   01噬菌体建库 从B淋巴细胞中扩增抗体的H链和L链基因文库，抗体片段群与噬菌体衣壳蛋白融合表达并展示于噬菌体表面，形成噬菌体展示抗体库。主要有三种类型： scFv库（由VH和VL组成，并由一个小肽（Gly4Ser）3连接成一个单链多肽）、Fab库（含有VH-CH，VL-CL