• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates

        互联网

        628
        All of the basic functional assays of complement activity utilize erythrocytes as targets (1 ). For classical pathway assays, the favored target is the antibody-sensitized sheep erythrocyte. For alternative pathway assays, unsensitized rabbit erythrocytes are routinely used. Numerous modifications on the basic assay methodology developed almost 50 years ago have found favor in different laboratories, which makes it difficult to compare results between laboratories. Some basic principles will be illustrated here and simple protocols for determination of hemolytic activities in the two major activation pathways (classical pathway CH50 and alternative pathway APH50) will be provided. It should be emphasized that measurements of total hemolytic complement provide only limited information. They are helpful as screening tests when complement deficiency is suspected (see Chapter 11 ) and can give a rather insensitive measure of complement activation. Despite this limited usefulness, hemolytic complement is often the only assay of complement activity available in the clinical laboratory. For accurate assessment of complement activity, serum samples for complement assay must be obtained fresh, promptly separated and either assayed immediately or stored frozen at −70�C until assay. Samples must not be subjected to freeze-thaw cycles.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序