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- 文献和实验
- 技术资料
- 英文名:
EpiQuik DNA Demethylase Activity/Inhibition Assay Core Kit (96 assays)
- 供应商:
上海西宝
- 规格:
96次分析
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文献和实验several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single?cell electrophoresis (comet assay), filter eluting, K
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
anti-DNA-POD (1: 5; 1: 10) overnight at 4°C. 7. Wash in PBS (3x5 min). 8. Demonstration of peroxidase activity in a Petri dish: dissolve a DAB tablet (10 mg) in 40 ml of 0.1M citrate acid-sodium citrate buffer (pH 5.5), add 5 ml of H2
) is then used to detect promoter specific transcripts (pRNAs) with the biotin linked pull-down assay (Figure 2) (comment 5);3.From the 200µl elute 30µl should be saved as input and the remaining 170µl from the extracted genomic DNA (Qiagen™ DNeasy) is mixed
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