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- 详细信息
- 文献和实验
- 技术资料
- 库存:
5 pk
- 英文名:
CAP™-GEL PLUS, TRIS-HYDROCHLORIDE
- 供应商:
西宝生物
- 保存条件:
0 TO 5 C
- 规格:
BIOCHEMICALS
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文献和实验ZOOM® IPGRunner™系统:简化的双向电泳(2D gel eletrophoresis)
入400μl 用合适的电泳缓冲液制备的0.5%琼脂糖溶液封盖。分子量标准(Mark12™ Unstained Protein Standard, Invitrogen, Cat. no. LC5677)上样到分子量标准孔。胶条使用NuPAGE® Novex 4-12% Bis-Tris ZOOM®胶(Invitrogen, Cat. no. NP0330),按照标准程序在XCell SureLock® Mini-Cell 槽(Invitrogen, Cat. no. EI001)中进行电泳。200
实验步骤 1. Protocol using XCell SureLock™ Mini-Cell 1) Remove the Novex® Pre-Cast Gel from the pouch. 2) Rinse the gel cassette with deionized water. Peel off the tape
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
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