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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
POLE2 Monoclonal Antibody [1A3] (100 ug)
- 规格:
100 ug
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文献和实验Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
If titre is positive do one of the following 2 weeks later: Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR Boost subcutaneously with 50 ug - 100 ug of Antigen in PBS and proceed
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