异绿原酸B 分析对照品

 本品为分析标准品。

使用本产品的案例（仅供参考）

HPLC

About 5 µL of the diluted AVTE sample solution was analyzed using an HPLC System and separated using an C18 column (2.6 µm, 4.6 × 150 mm). Mobile phase A was water containing 0.5% acetic acid, and mobile phase B was acetonitrile. The flow rate was 0.5 mL/min with a column temperature of 30℃, and the detection wavelength was 328 nm. The gradient elution conditions were as follows: Equilibrium stage was set for 10 min with 12% B (isocratic) then, 0–30 min with 12%–45% B (linear gradient), 30–35 min with 45%–100% B (linear gradient), and 35–40 min with 100% B (isocratic).

来源文献：Li C, Tan F, Yang J, Yang Y, Gou Y, Li S, Zhao X. Antioxidant Effects of Apocynum venetum Tea Extracts on d-Galactose-Induced Aging Model in Mice. Antioxidants (Basel). 2019 Sep 8;8(9):381. doi: 10.3390/antiox8090381. PMID: 31500342; PMCID: PMC6770887.

UHPLC

Chromatographic separation of SPLFA and SPLF was done on an Agilent 1290 UHPLC system  coupled to an auto sampler, a diode array detector (DAD) and an ACQUITY UHPLC® HSS T3 column (2.1 ×100 mm, 1.8 μm; Waters, Milford, MA, USA)maintained at 30 ◦C. Eluent A was 0.05% formic acid in water and eluent B was 0.05% formic acid in acetonitrile. Elution of the SPLPA compounds was achieved using the following linear gradient elution (in %B): 0 min, 5%; 20 min, 60%; 20.1 min, 5%; 30 min, 5%, and for the SPLF compounds: 0 min, 20%, 25 min, 50%, 25.1 min, 20%, 35 min, 20%. The injection volume was 5 μL, with a flow rate of 0.3 mL/ min. All sample solutions were filtered through a 0.22 μm PTFE filter (Agilent Technologies) before analysis. The detection wavelength was 320 nm for SPLPA and 254 nm for SPLF. 

来源文献：[1] Luo D , Mu T , Sun H .Profiling of phenolic acids and flavonoids in sweet potato (Ipomoea batatas L.) leaves and evaluation of their anti-oxidant and hypoglycemic activities[J].Food Bioscience, 2020, 39(44):100801.DOI:10.1016/j.fbio.2020.100801.

UPLC

The standards arbutin, (+)-catechin, (−)-epicatechin, neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, rutin, oleanolic, ursolic, p-coumaric, caffeic and quinic acid were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Luteoloside, kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and hyperoside were bought from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China).

来源文献：Sun L, Tao S, Zhang S. Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS. Molecules. 2019 Jan 3;24(1):159. doi: 10.3390/molecules24010159. PMID: 30609827; PMCID: PMC6337724.