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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
SIRT3 Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg
蛋白名称:SIRT3 Protein
蛋白构建:Recombinant full length human SIRT3 was expressed in E. coli cells using an N-terminal GST tag.
表达宿主:E. coli
蛋白分子量:~66 kDa
蛋白NP号:NM_012239
蛋白氨基酸序列:Full Length
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文献和实验Oxygen Flux Analysis to Understand the Biological Function of Sirtuins
The sirtuins are a family of highly conserved NAD+ -dependent lysine deacylases with important roles in metabolic regulation. Of the seven mammalian sirtuins, three localize to the mitochondria: SIRT3, SIRT4, and SIRT5. Mitochondrial sirtuins
Chemical Acetylation and Deacetylation
acetylation on protein lysine residues is characterized by native gel electrophoresis and Western blotting. Acetyl-Lys modifications are then examined for deacetylation using a SIRT3 deacetylase activity assay and followed by stable isotope dilution mass
In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
Evaluating the precise catalytic activity of sirtuin proteins in vivo is a challenging endeavor. Enzymological methods, including those employed in commercially available kits, require the isolation of immunopurified protein from cells
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