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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fusion protein of Human PRMT7
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
/
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
Q9NVM4
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA,WB;ELISA:1:2000-1:5000,WB:1:500-1:2000
- 浓度:
>95%,Antigen affinity purification
- 靶点:
PRMT7
- 抗体英文名:
PRMT7 Antibody
- 抗体名:
Protein arginine N-methyltransferase 7 antibody
- 规格:
100μl/50μl
| 规格: | 100μl | 产品价格: | ¥1980.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥1100.0 |
保存缓冲液
-20℃, pH7.4 PBS, 0.05% NaN3, 40% Glycerol功能
Protein arginine methyltransferase 7 is a protein that in humans is encoded by the PRMT7 gene. Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing. May be involved in etoposide-induced cytotoxicity, a chemotherapeutic agent frequently used for testicular cancer and small-cell lung cancer that can cause cytotoxicity in the treatment of other cancers. Down-regulation confers increased sensitivity to the Top1 inhibitor camptothecin (CPT).风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验基因工程抗体(genetically engineered antibody)
蛋白质酶解、 折叠和糖基化等修饰过程。酵母的生长速度(1 ~3 小时/代)比动物细胞快(约1 天/代),营养条件比 大肠杆菌更简单。并且,其所表达的蛋白质大都以可溶性形式分泌到培养基中,有利于后期纯化。目 前应用最多的是酿酒酵母和毕赤酵母,后者具有乙醇氧化酶基因(AOXI),在甲醇的存在下能迅速启动 转录,所以更适合高密度培养。酵母具有与高等真核生物类似的O -糖基化和N -糖基化,但是酵母的 糖链成分主要是甘露糖,并且糖链过长,这种过度糖基化有可能会影响抗体的空间结构,进而影响抗体的活性。有报道
Typical modification sites: RGG box or RXR sequence motifs R-arginine, G-glycine, X-any aminoacid. Enzymes catalysing protein arginine methylation: PRMT1 from rat and the human homologue HRMT1L2, human PRMT2 (HRMT1L1), rat and human PRMT
monoclonal antibody production fusion
, 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml 上一篇:Antibody Conjugation Protocol 下一篇:Expanding Hybridoma Clones
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