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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Collagenase, Type II
- 供应商:
MP Biomedicals
- 保存条件:
2-8 ℃
- 规格:
02100502.3(25mg)/02100502.1(100 mg)/02100502.5(500mg)/0210050201(1 g)/0210050205(5 g)
| 规格: | 02100502.3(25mg) | 产品价格: | ¥557.42 |
|---|---|---|---|
| 规格: | 02100502.1(100 mg) | 产品价格: | ¥938.71 |
| 规格: | 02100502.5(500mg) | 产品价格: | ¥3040.39 |
| 规格: | 0210050201(1 g) | 产品价格: | ¥4902.62 |
| 规格: | 0210050205(5 g) | 产品价格: | ¥23585.52 |
Collagenase used for collagen structural and biosynthetic studies. For tissue dissociation most researchers employ either crude collagenase preparations or chromatographically purified collagenase combined with other enzymes such as elastase, trypsin, and/or papain. Collagenase from Clostridium histolyticum has been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase. Collagenase from Clostridium histolyticum has also been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans.
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文献和实验1.Pang L, Dunterman M, Xuan W, et al. Circadian regulator CLOCK promotes tumor angiogenesis in glioblastoma. Cell Rep. 2023;42(2):112127. doi:10.1016/j.celrep.2023.112127
2.Sandoval K, Berg MD, Guadagnin AR, Cardoso FC, Dean M. Endometrial glycogen metabolism on days 1 and 11 of the reproductive cycle in dairy cows. Anim Reprod Sci. Published online August 20, 2021. doi:10.1016/j.anireprosci.2021.106827
3.Andueza A, Kumar S, Kim J, et al. Endothelial Reprogramming by Disturbed Flow Revealed by Single-Cell RNA and Chromatin Accessibility Study. Cell Rep. 2020;33(11):108491. doi:10.1016/j.celrep.2020.108491
科镊将该组织一端固定,眼科镊与该组织垂直,并夹持整个横截面,术者同法夹持于附近,助手持第3把眼科镊提起肌组织,术者以手术刀锐性分离肌层;如此,向另一端推进,锐性分离肌条。6、在超净台,取相当于0.5为X0.5 CM2肌块,室温下将其剪碎成1~2mm碎组织块7、转移入离心管8、加入D-hanks液(操作成功) OR Dulbecco9、离心1000转/min,10分钟 离心半径13cm?弃上清10、加入I型胶原酶(2mg/ml) 5mg,(II型胶原酶2mg/ml 5mg也可,但效果不如I型胶原酶)11
) ● 培养液:RPMI1640 培养液+10%AB 型血清+IL-2(200u/ml)或X-VIVO15 培养液 ● 消化液:IV 型胶原酶(230u/g)、I 型DNA 酶3600u 、V 型透明质酸酶(1500u/g) ● 器皿与仪器:75cm2 培养瓶,15ml 聚丙烯试管,手术刀、剪、镊,CO 2 孵箱,三角烧瓶,磁力搅拌器等 实验操作 1.在无菌条件下将切除新鲜瘤体组织去除坏死部分与结缔组织,用RPMI1640 培养液冲洗干净
on ice 2. Add 5 - 50 µl (0.5 to 5 mCi) 125I using microsyringe 3. Add 50 µl of lactoperoxidase (1 mg/ml) 4. Add 10 µl of glucose oxidase (5 U/ml) 5. on ice, 5 min (occasionally agitate) 6. Add additional 10 µl of glucose oxidase (5 U/ml)











