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- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human Myelin and lymphocyte protein (1-84AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Upon receipt, store at -20℃ or -80℃. Avoid repeated freeze.
- 克隆性:
Polyclonal
- 标记物:
Non-conjugated
- 适应物种:
Human
- 保质期:
6个月
- 抗原来源:
Homo sapiens (Human)
- 目录编号:
P21145
- 级别:
优
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
- 浓度:
>95%,Protein G purified
- 靶点:
MAL
- 抗体英文名:
MAL Antibody
- 抗体名:
MAL;Myelin and lymphocyte protein;T-lymphocyte maturation-associated protein
- 规格:
100μg/50μg/20μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
| 规格: | 20μg | 产品价格: | ¥440.0 |
保存缓冲液
Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
功能
Could be an important component in vesicular trafficking cycling between the Golgi complex and the apical plasma membrane. Could be involved in myelin biogenesis and/or myelin function.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验-maleimide 染料与膜蛋白冰上孵育2. preclear:加入 control IgG 和 protein A/G beads,孵育,离心,吸取上清3. IP:先后加入抗体和 protein A/G beads 孵育,离心,保留 beads4. RIPA 清洗 beads,DTT 处理5. 煮样,蛋白电泳,转印6. 采集荧光信号——激光扫描7. 封闭,一抗孵育,HRP 二抗孵育8. 采集化学发光信号——胶片显影 小编:如果那个时候有 iBright,就可以同时进行成像了。条带获取更容易,且对比数据更具代表
Antibody Affinity Maturation by Random Mutagenesis
consists of somatic hypermutation of V-region genes followed by clonal selection of B-cells that produce antibodies of the highest affinity. Recent advances in phage-displayed antibody technology have enabled the affinity maturation process to be mimicked
Antibody Affinity Maturation by Chain Shuffling
Phage display can be used to increase the affinity of antibodies more than 1,000-fold (1 ,2 ). The starting point is typically a specific antibody isolated from a phage antibody library (see Chapter 8 ). To accomplish affinity maturation
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