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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Suregada multiflora Ribosome-inactivating protein gelonin (GEL)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥17754.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥4956.0 |
| 规格: | 20μg | 产品价格: | ¥2616.0 |
纯度:
Greater than 85% as determined by SDS-PAGE.基因名:
GELUniprot No.:
P33186别名:
rRNA N-glycosidase;allergen Gel m RIP种属:
Suregada multiflora (False lime) (Gelonium multiflorum)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
29.7 kDa表达区域:
47-297aa氨基酸序列:
GLDTVSFSTKGATYITYVNFLNELRVKLKPEGNSHGIPLLRKKCDDPGKCFVLVALSNDNGQLAEIAIDVTSVYVVGYQVRNRSYFFKDAPDAAYEGLFKNTIKTRLHFGGSYPSLEGEKAYRETTDLGIEPLRIGIKKLDENAIDNYKPTEIASSLLVVIQMVSEAARFTFIENQIRNNFQQRIRPANNTISLENKWGKLSFQIRTSGANGMFSEAVELERANGKKYYVTAVDQVKPKIALLKFVDKDPK蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
/内毒素:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
Developed ProteinBiological_Activity:
/Research Areas:
OthersReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
/Function:
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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
Figure 4.18.1 Representative gels from intermediate product purification. (A ) Size selection of ribosome footprint fragments. The footprinting samples are derived from HeLa lysates with 5 to 15 µg input RNA. The blue bracket indicates the gel
Synchronizing Protein Transport in the Secretory Pathway
topologies. (A ) The hook is placed upstream of the IRES (internal ribosome entry site) while the reporter is downstream of it. The four cloning cassettes (A to D) enable cloning of the different domains necessary to create a reporter fusion protein
A Method for Assaying Deubiquitinating Enzymes
for ribosome biogenesis as well as of free Ubs. Deubiquitinating enzymes (DUBs) are known to consist of a large protein family in eukaryotes. For example, the budding yeast has 17 genes for DUBs (4 ). Moreover, so far more than 60 full-length DUB sequences
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