相关产品推荐更多 >

Recombinant-Pongo-abelii-Atlastin-1ATL1Atlastin-1 EC= 3.6.5.-
¥13734
Recombinant-Methanocaldococcus-jannaschii-Uncharacterized-protein-MJ0027MJ0027Uncharacterized protein MJ0027
¥10416
Recombinant-Escherichia-coli-Lipid-A-biosynthesis-lauroyl-acyltransferasehtrBLipid A biosynthesis lauroyl acyltransferase EC= 2.3.1.- Alternative name(s): Heat shock protein B
¥11494
Recombinant-Human-Olfactory-receptor-4C15OR4C15Olfactory receptor 4C15 Alternative name(s): Olfactory receptor OR11-127 Olfactory receptor OR11-134
¥11592
Recombinant-Saccharomyces-cerevisiae-Glutathione-transferase-3GTT3Glutathione transferase 3
¥11774
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
细胞污染识别与处理全攻略:5 种常见类型+关键误区abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Stenotrophomonas maltophilia Chaperonin GroEL (groEL), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Stenotrophomonas maltophilia Chaperonin GroEL (groEL), partial品名简称:
Recombinant Stenotrophomonas maltophilia groEL protein, partial货号:
CSB-EP474378FLY规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
groELUniprot No.:
B4SKL8别名:
60 kDa chaperonin;Chaperonin-60;Cpn60种属:
Stenotrophomonas maltophilia (strain R551-3)蛋白长度:
Partial来源:
E.coli分子量:
61.6 kDa表达区域:
4-532aa氨基酸序列:
KDIRFGEDARSRMVRGVNVLANAVKATLGPKGRNVVLEKSFGAPTITKDGVSVAKEIELADKFENMGAQMVKEVASRTNDDAGDGTTTATVLAQALIREGAKAVAAGMNPMDLKRGIDKAVVAAVNELKSISKPTADDKAIAQVGTISANSDESIGQIIADAMKEVGKEGVITVEEGSGLDNELDVVKGMQFDRGYLSPYFINNQQSQTADLDDPFILLHDKKISNVRDLLPVLEGVAKAGKPLLIVAEEVEGEALATLVVNTIRGIVKVVAVKAPGFGDRRKAMLEDMAVLTGGTVISEEVGLSLEKATIKDLGRAKKVQVSKENTTIIDGVGDKAAVDSRVAQIKTQIQDTSSDYDREKLQERVAKLAGGVAVIKVGASTEIEMKEKKDRVDDALHATRAAVEEGVVPGGGVALVRAVSALAGLKGANEDQNHGIQIALRAMEAPLREIVANAGEEPSVIVNKVKEGTGSFGYNAATGEFGDMLQFGILDPTKVTRSALQNAASIAGLMITTEAMVAEAPKKDEPAM蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.功能2:
/文献:
/研究领域:
Others请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Strategies to Optimize Protein Expression in E. coli
Amada, K., Yohda, M., Odaka, M., Endo, I., Ishii, N., Taguchi, H., and Yoshida, M. 1995. Molecular‐cloning, expression, and characterization of Chaperonin‐60 and Chaperonin‐10 from a thermophilic bacterium
包涵体表面吸附的大部分不溶性杂蛋白。洗涤液pH以与工程菌生理条件相近为宜,使用的还原剂为0.1-5mM。EDTA为0.1-0.3 mM。去垢剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 1.2.5 DNA片段分析 SGC-7901感染Ad-p27mt和Ad-LacZ 48 h后离心去上清, 在剩余细胞
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨
技术资料暂无技术资料 索取技术资料






