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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Synaptosomal-associated protein 25 (Snap25)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Synaptosomal-associated protein 25 (Snap25)品名简称:
Recombinant Mouse Snap25 protein货号:
CSB-EP021873MOe1规格:
20μg价格:
2340LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Snap25Uniprot No.:
P60879别名:
SNAP-25;Super protein;SUP;Synaptosomal-associated 25 kDa protein种属:
Mus musculus (Mouse)蛋白长度:
Full Length来源:
E.coli分子量:
23.2 kDa表达区域:
1-206aa氨基酸序列:
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG蛋白标签:
Tag-Free产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.功能2:
/文献:
/研究领域:
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文献和实验protein of Mr 25 kDa [SNAP-25] which are also known under the collective term of SNAREs) involved in docking and fusion of synaptic vesicles at the active zone (i.e., release site) (for reviews see [1 ,2 ]).
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31.25-2000pg/ml EU3598 MSTN ELISA Kit 18.75pg/ml 31.25-2000pg/ml 重组蛋白&抗体 货号 产品名称 宿主 分子量/应用 P0302 Recombinant Human GDF-8 E.Coli 33.3 kDa Pr22272 Recombinant Mouse Latent
在Journal of Biological Chemistry在线发表了一篇研究。该研究发现E3泛素连接酶TRIM25通过催化HIF-1α与HIF-2α关键赖氨酸位点发生K11连接的多聚泛素化修饰,从而稳定其蛋白水平,增强低氧信号通路并促进低氧耐受。功能实验表明,trim25敲除的斑马鱼在低氧胁迫下红细胞数量更少、死亡率更高,Trim25敲除小鼠血清Epo含量更低且对低氧更敏感。机制上,TRIM25结合HIF-1α介导其K719和K721位点、以及结合HIF-2α催化其K709位点发生K11连接的多聚泛素
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