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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human HLA-DRB1&HLA-DRA Heterodimer Protein-VLPs
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥36252.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥6840.0 |
| 规格: | 20μg | 产品价格: | ¥3420.0 |
纯度:
The purity information is not available for VLPs proteins.基因名:
HLA-DRB1&HLA-DRAUniprot No.:
D7RIG5&P01903别名:
/种属:
Homo sapiens (Human)蛋白长度:
Heterodimer来源:
Mammalian cell分子量:
28.3 kDa&27.0 kDa表达区域:
30-266aa&26-254aa氨基酸序列:
GDTQPRFLWQGKYKCHFFNGTERVQFLERLFYNQEEFVRFDSDVGEYRAVTELGRPVAESWNSQKDILEDRRGQVDTVCRHNYGVGESFTVQRRVHPEVTVYPAKTQPLQHHNLLVCSVSGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSVMSPLTVEWRARSESAQSKMLSGVGGFVLGLLFLGAGLFIYFRNQKGHSGLQPTGFLS&IKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENVVCALGLTVGLVGIIIGTIFIIKGVRKSNAAERRGPL蛋白标签:
C-terminal 10xHis-tagged & C-terminal Flag-tagged (This tag can be tested only under denaturing conditions)产品提供形式:
Lyophilized powder缓冲液:
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4.货期:
18-28 business days注意事项:
The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).功能:
/内毒素:
Not TestSDS-PAGE:
CSB-MP5177HU is detected by Mouse anti-6*His monoclonal antibody.LC-MS Image Description/Western Blot:
/Product types:
MP-VLP Transmembrane Protein & Developed ProteinBiological_Activity:
/Research Areas:
ImmunologyReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.Reference:
/Function:
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文献和实验Clonality - X Chromosome Inactivation Assay
and can assist in interpretation of allele patterns. 4. References Allen RC, Zoghbi HY, Moseley AB, Rosenblatt HM, Belmont JW. Methylation of HpaII and HhaI sites near the polymorphic CAG repeat in the human androgen-receptor gene correlates with X chromosome
在人细胞中快速、高水平地表达目的蛋白 在2至3个星期内即可获得高效价的病毒 无需噬斑纯化 靶细胞可以是分裂或不分裂的细胞(dividing/or nondividing cell) CLONTECH的Adeno-X™表达系统是一个特别 高效的腺病毒表达系统,用于在人细胞中瞬时及高水平地表达目的蛋白质。由于Adeno-X™病毒DNA中含有特别设计的克隆位点,你可以在2星期内获得高效价的腺病毒,其效率远高于其他腺病毒系统。 高效表达目的蛋白
了解一下。 实验目的:测定肝癌细胞 X 基因相对于正常肝细胞的表达量。通过荧光定量检测可得:肝癌细胞中 X 基因的 CT= 25,正常肝细胞中 X 基因的 CT= 26,所以,利用表达量倍数计算公式 2-△CT计算,发现肝癌细胞中的 X 基因表达量是正常肝细胞表达量的 2 倍。 但是,上面这个定量结果检测有效的前提是:必须保证两盘细胞的细胞数量完全一致;RNA 提取、逆转录以及定量效率及操作必须完全一致;不存在操作误差等。这显然是无法达到的。 那如何才能使这些误差不影响 X 基因
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