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CHRM5/CHRM5蛋白Recombinant Human

Muscarinic acetylcholine receptor M5 (CHRM5)重组蛋白/蛋白
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  • ¥9720 - 16200
  • CUSABIO已认证
  • CN
  • CSB-CF005385HU
  • 2025年07月12日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human Muscarinic acetylcholine receptor M5 (CHRM5)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      100μg/20μg

    规格:100μg产品价格:¥16200.0
    规格:20μg产品价格:¥9720.0

    纯度:

    Greater than 95% as determined by SDS-PAGE.

    基因名:

    CHRM5

    Uniprot No.:

    P08912

    别名:

    /

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Full Length

    来源:

    in vitro E.coli expression system

    分子量:

    66.1 kDa

    表达区域:

    1-532aa

    氨基酸序列:

    MEGDSYHNATTVNGTPVNHQPLERHRLWEVITIAAVTAVVSLITIVGNVLVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTTYILMGRWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLISFILWAPAILCWQYLVGKRTVPLDECQIQFLSEPTITFGTAIAAFYIPVSVMTILYCRIYRETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLAQRERNQASWSSSRRSTSTTGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPATDPVLQVVYKSQGKESPGEEFSAEETEETFVKAETEKSDYDTPNYLLSPAAAHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMTKRKRVVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTVNPICYALCNRTFRKTFKMLLLCRWKKKKVEEKLYWQGNSKLP

    蛋白标签:

    N-terminal 10xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    18-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

    内毒素:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    CF Transmembrane Protein & Developed Protein

    Biological_Activity:

    /

    Research Areas:

    Neuroscience

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    /

    Function:

    /

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    图标文献和实验
    该产品被引用文献
    更多文献信息可以查看cusabio官网
    相关实验
    • Overview of Receptor Allosterism

      Figure 1.21.1 Allosterism between a receptor and its G protein: the effects of GTP on the competition between [3 H] N ‐methylscopolamine and the agonist, carbachol, at the muscarinic acetylcholine M2 receptor in rat myocardial membranes. Competition

    • 化学遗传学技术原理及其应用

      DREADDs受体作用原理(Scott M. Sternson & Bryan L. Roth, Annu. Rev. Neurosci., 2014)2. 常见的DREADDs受体(1)Gq-DREADD和hM3Dq:最初的Gq-DREADD即hM3Dq,改造自人毒蕈碱型乙酰胆碱受体(the human muscarinic acetylcholine receptor,mAchRs)亚型M3(也称为hM3)。在正常生理状况下,hM3和乙酰胆碱结合,然后和Gq类G蛋白耦合受体耦合,作用于磷脂酶C、肌醇三磷酸

    • Measuring Receptor Target Coverage: A Radioligand Competition Binding Protocol for Assessing the Association and Dissociation Rates of Unlabeled Compo

      Figure 9.14.6 Saturation data for the binding of [3 H]NMS to the M3 muscarinic receptor. The mean bound cpm is plotted against the amount of added radioligand (radioligand concentration). Specific binding is the difference between total

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    文献支持
    CHRM5/CHRM5蛋白Recombinant Human Muscarinic acetylcholine receptor M5 (CHRM5)重组蛋白/蛋白
    ¥9720 - 16200